人原发性肝癌胰岛素样生长因子2和H19基因印迹研究  

The Aberrant Imprinting of Insulin-like Growth Factor Ⅱ and H19 in Human Hepatocellular Carcinoma

作　　者：武静[1] 覃扬[1] 李波[2] 何文智[1] 孙芝琳[1] 

机构地区：[1]四川大学华西基础医学与法医学院生物化学与分子生物学教研室,成都610041 [2]四川大学华西医院普外科

出　　处：《四川大学学报（医学版）》2007年第1期49-52,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30470792)资助

摘　　要：目的研究人原发性肝癌中胰岛素样生长因子2(insu lin-like grow th factorⅡ,IGF 2)和H 19基因的印迹状态,为进一步研究印迹调控机制打下基础。方法采用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)筛选IGF 2和H 19基因杂合标本,并用逆转录PCR-RFLP检测杂合标本IGF 2和H 19基因的印迹状态。结果正常人肝组织中,IGF 2呈双等位基因表达,H 19呈单等位基因表达;47.1%(8/17)的肝癌标本中检测到IGF 2基因的印迹获得,45.5%(5/10)的肝癌标本中检测到H 19基因的印迹丢失;IGF 2和H 19的印迹改变没有相关性。结论IGF 2和H 19的印迹异常与肝癌有关,成人肝组织中IGF 2与H 19的印迹调控可能相对独立。Objective To investigate the allelie expression of IGF2/H19 in human hepatoeellular carcinoma and to analyze the relationship between the imprint status of IGF2 and that of H19. Methods Heterozygotes of IGF2 and of H19 were identified by restriction fragment length polymorphism （RFLP）. Allelie expression was detected by reverse transcription-polymerase chain reaction （RT-PCR） and RFLP. Results Forty seven point one percent （47.1%, 8 of 17） of HCC samples were demonstrated to have the gain of imprinting （GOI） of in IGF2 gene, and 45.5% （5 of 11） of HCC samples were found to have the loss of imprinting （LOI） for H19 gene. No relationship was observed between the imprinting status of IGF2 and that of H19. Conclusions IGF2 GOI and H19 LOI are common in HCC, and the imprinting of IGF2 could be independent from that of H19 in adult liver.

关 键 词：H19 胰岛素样生长因子2 人原发性肝癌 基因组印迹 

分 类 号：R735.7[医药卫生—肿瘤]