马桑内酯对大鼠海马神经元钠电流的影响  

Effects of Coriaria Lactone on the Sodium Currents of Actuely Isolated Rat Hippocampal Neurons

作　　者：张勤[1] 赖晓晖[1] 廖大清[2] 刘洁[3] 周东[1] 

机构地区：[1]四川大学华西医院神经内科,成都610041 [2]四川大学麻醉与危重急救研究室 [3]四川省人民医院神经内科

出　　处：《四川大学学报（医学版）》2007年第1期119-122,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30370513)资助

摘　　要：目的研究马桑内酯(coriaria lactone,CL)对大鼠海马神经元钠离子通道电流的影响,探讨该影响在CL致痫中的意义。方法利用全细胞膜片钳技术,在急性分离的大鼠海马神经元上记录钠电流,观察CL对电流幅度的影响。结果经0.1 m g/mL、0.2 m g/mL的CL作用后,海马神经元钠电流有不同程度的增加〔CL 0.1 m g/mL组的最大峰值电流密度为(-90.11±14.02)pA/pF,增幅为17.32%±8.52%;CL 0.2 m g/mL组的最大峰值电流密度为(-111.52±6.65)pA/pF,增幅为37.98%±4.91%〕;经与对照组相比,CL 0.2 m g/mL组(P<0.05)的变化较CL 0.1 m g/mL组(P>0.05)明显。结论CL使海马神经元电压依赖性钠电流的幅度增大,从而改变细胞的兴奋性,引发异常放电。Objective The present study tried to find out the effeets of eoriaria laetone （CL） on the sodium currents of the rat hippocampal neurons and their significance. Methods The sodium currents of the acutely isolated Sprague-Dawley rat hippocampal neurons were recorded by employing the patch-clamp technique in the whole-eell voltage-clamp mode. The effects of CL on the peak currents in the neuron membrane were assessed and analyzed. Results Both 0. 1 mg/mL and 0. 2 mg/mL CL inereased the maximal peak density of sodium eurrents signifieantly （17. 32%±8. 52% vs 37. 98%±4. 91%）. The effeet of 0. 2 mg/mL CL （P〈0. 05） was more signifieant than that of 0. 1 mg/mL CL （P〉0. 05）, when eompared with the control （3. 16%±0. 65%）. Conclusion CL can increase the amplitudes of the voltage-dependent sodium currents in the acutely isolated hippocampal neurons. This effect may contribute to the enhanced excitability of hippocampal neurons and play a role in the epileptogenetic process of CL.

关 键 词：马桑内酯 海马神经元 钠电流 膜片钳技术 癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学]