人重组粒细胞集落刺激因子治疗大鼠缺血性脑梗死的实验研究  

The study of recombinant human granulocyte colony-stimulating factor in the treatment of ishemic cerebral infarction in rat

作　　者：刘娜[1] 张俊[1] 陈大方[2] 樊东升[1] 张琳 

机构地区：[1]北京大学第三医院神经科,100083 [2]北京大学医学部公共卫生学院流行病学与卫生统计学系 [3]吉林省吉林市5704厂职工医院

出　　处：《北京医学》2007年第1期1-3,共3页Beijing Medical Journal

基　　金：首都医学发展科研基金资助项目(2002-3006)

摘　　要：目的探讨人重组粒细胞集落刺激因子(rhG-CSF)对大鼠缺血性脑梗死的治疗作用及其应用的时间窗。方法采用线栓法制作大鼠缺血性脑梗死模型,分别于梗死后1h、6h给予皮下注射rhG-CSF(50μg/kg);对照组给予同等剂量的生理盐水皮下注射。TTC染色检测梗死体积。结果术后48h处死大鼠,梗死后1h给药,治疗组与对照组梗死体积分别为(135.0±13.8)mm3、(230.0±57.8)mm3,两者比较P<0.001;梗死后6h给药,梗死体积分别为(140.0±25.3)mm3、(240.0±30.6)mm3,两者比较P<0.001。结论rhG-CSF可减轻大鼠缺血性脑梗死程度,减小梗死体积。术后6h给药与术后1h给药具有相当的疗效。Objective To explore whether recombinant human granulocyte colony-stimulating factor（rhG-CSF） can be used to treat ischemic cerebral infarction in rats and the time for treatment. Methods Rat cerebral infarction model was established by line occlusion. The rats received rhG-CSF（50 μg/kg, subcutaneously） or vehicle（saline） at 1 hour and 6 hours after ischemia. The technique of TYC staining was used to detect the volume of infarction. Results The infarction volume of the rat brain was smaller in the two rhG-CSF therapy groups than the two control groups 48 hours later（P〈0.01）. There was no difference between the two treatment groups. Conclusions rhG-CSF can relieve the degree of ischemia and reduce the infarction volume. The efficacy of treating at 6 hours after ischemia is the same as treating at lhour after ischemia.

关 键 词：人重组粒细胞集落刺激因子 大脑中动脉梗死 治疗 

分 类 号：R743.3[医药卫生—神经病学与精神病学]