高速逆流色谱分离制备丹参脂溶性成分  

Preparation of Tanshinones from Salvia Miltiorrhiza Bunge by HSCCC

作　　者：屈燚[1] 杨悦武 郭治昕[2] 祝国光[2] 

机构地区：[1]天津大学化工学院,天津300072 [2]天士力集团有限公司,天津300402

出　　处：《化学工业与工程》2007年第1期48-51,共4页Chemical Industry and Engineering

摘　　要：应用高速逆流色谱法(High-Speed Counter-current Chromatography,HSCCC),从丹参的乙醇粗提物中分离纯化脂溶性成分。试验研究建立了以石油醚-乙酸乙酯-甲醇-水为溶剂体系,并优化了HSCCC的操作参数及操作方法,快速有效地分离得到5个丹参酮类化合物。经HPLC方法测定,其中丹参酮ⅡA、隐丹参酮、丹参酮Ⅰ、二氢丹参酮Ⅰ纯度均大于98%。该方法操作简捷,快速高效、费用低廉,重现性好,为中药丹参的质量控制所需要的高纯度化学对照品,提供了一种优良的制备分离方法。Separation and preparation of tanshinones from Danshen （Salvia miltiorrhiza Bunge ） crude ethanol extract were studied by high-speed counter-current chromatography （HSCCC）, The solvent system of petroleum ether-ethyl acetate-methanol-water （8：6：7：3） was selected and the optimum running parameters for apparatus was obtained through orthogonal experiment. Five tanshinone were separated and identified rapidly. Measured by HPLC, the purity of tanshinone Ⅱ A, cryptotanshinone, tanshinone Ⅰ , dihydrotanshinone Ⅰ were all over 98%. HSCCC is simple, economical, quick and with good repeatability for preparation of Danshen active components.

关 键 词：高速逆流色谱 丹参 丹参酮 

分 类 号：TQ028.8[化学工程]