Antisense expression of a rice cellular apoptosis susceptibility gene (OsCAS} alters the height of transgenic rice  

作　　者：XU Chunxiao HE Chaozu 

机构地区：[1]National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China [2]Graduate University of Chinese Academy of Sciences, Beijing 100039, China [3]Shenzhen Laboratory of Superhybrid Rice Research, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China

出　　处：《Progress in Natural Science:Materials International》2007年第1期39-43,共5页自然科学进展·国际材料（英文版）

基　　金：Supported by National Program oil Key Basic Research Projects(Grant No.2006CBl01900)

摘　　要：Cellular apoptosis susceptibility （CAS） gene plays important roles in mitosis, development and export of importin a from the nucleus, but its function in plant is unknown. In this study, a rice CAS ortholog （ OsCAS ）, which encodes a predicted protein of 983 amino acids with 62 % similarity to human CAS, was identified. DNA gel blot analysis revealed a single copy of OsCAS in the rice genome. A 973 by fragment at the 3 end of OsCAS eDNA was cloned from rice eDNA library and transferred into rice in the antisense direction under the control of CaMV 35S promoter via Agrobacterium-mediated transformation method, 105 transgenic lines were obtained. Expression of OsCAS was suppressed in the antisense transgenic lines as revealed by semi-quantitative RT-PCR. The antisense transgenic lines showed dwarf phenotypes. The reanlts indicated that OsCAS was involved in culm development of rice.

关 键 词：ANTISENSE DWARF ORTHOLOG OsCAS rice 

分 类 号：S511[农业科学—作物学]