Application of synchrotron source based DEI method in guinea pig cochleae imaging  

作　　者：YIN Hongxia LIU Bo GAO Xin GAO Xiulai LUO Shuqian 

机构地区：[1]College of Biomedical Engineering, Capital University of Medical Sciences, Beijing 100069, China [2]Department of Anatomy, Capital University of Medical Sciences, Beijing 100069, China

出　　处：《Progress in Natural Science:Materials International》2007年第1期59-67,共9页自然科学进展·国际材料（英文版）

基　　金：Supported by National Natural Science Foundation of China(Grant No.60532090);Beijing Natural Science Foundation(No.7031001)

摘　　要：Hard X-ray diffraction enhanced imaging （DEI）, which is based on a synchrotron source and monochromator-analyzercrystal system, is an effective method for imaging X-ray phase shift. Utilizing an analyzer crystal with high angular sensitivity of micro-radian, DEI can measure the transmitted, refracted and scattered X-rays when projecting onto a sample. It dramatically improves the contrast and spatial resolution of the resultant images. At the topography station of Beijing Synchrotron Radiation Facilities （BSRF）, we implemented DEI method in guinea pig cochleae imaging and acquired a series of DEI images. Based on these images, the apparent absorption and refraction images were calculated. The DEI images revealed the holistic spiral structures and inner details of guinea pig cochleae clearly, even including the structures at the cellular level, such as the static cilia of hairy cells and the limbns of Hansen cell. Due to the advantages of high contrast, high spatial resolution and distinct edge-enhanced effect, DEI method promises extensive applications in biology, medicine and clinic in the near future.难 X 光检查衍射提高的成像(DEI ) 基于同步加速器来源和 monochromator-analyzer-crystal 系统，是为成像的一个有效方法 X 光检查阶段移动。与高尖的敏感利用分析器晶体微弧度， DEI 罐头措施播送，测定并且当投射到一件样品上时，散布了 X 光。它戏剧性地改进对比和结果的图象的空间分辨率。在北京同步加速器放射设备(BSRF ) 的地形学车站，我们在畿尼猪 cochleae 成像实现了 DEI 方法并且获得了一系列 DEI 图象。把图象，明显的吸收和折射基于这些图象被计算。DEI 图象清楚地揭示了整体的螺线结构和畿尼猪 cochleae 的内部细节，甚至包括在细胞的水平的结构，例如毛乎乎的房间的静态的睫和汉森房间的异色边缘。由于高对比，高空间的分辨率和不同提高边的效果的优点， DEI 方法在不久的将来在生物学，药和诊所答应广泛的应用程序。

关 键 词：diffraction enhanced imaging hard X-ray imaging phase-contrast imaging COCHLEA MICRO-STRUCTURE synchrotron-source 

分 类 号：R318.6[医药卫生—生物医学工程]