忍冬属植物花药药隔的特化结构及其生物学意义  

Morphological characteristics and biological significance of specialized connectives in Lonicera (Caprifoliaceae)

作　　者：江幸山[1] 吴鸿[1] 伦璇[2] 陆东雯[2] 

机构地区：[1]华南农业大学生命科学学院,广州510642 [2]华南农业大学测试中心,广州510642

出　　处：《植物分类学报》2007年第1期39-51,共13页Acta Phytotaxonomica Sinica

基　　金：国家自然科学基金项目(30670119)~~

摘　　要：花药药隔的特化结构通常具有重要的生物学意义。目前,有关药隔特化出贮存酚类物质的异细胞群的研究报道甚少,该特化结构有何生物学意义则未见报道。本研究运用半薄切片技术、冰冻切片技术、组织化学和细胞化学等方法对忍冬属Lonicera植物特化药隔异细胞群的主要内含物进行定性分析,实验证明忍冬属特化药隔异细胞群内含丰富的酚类物质。另外通过对忍冬属华南忍冬L.confusa药隔薄壁组织的发育及其与药隔维管束的关系分析,推测忍冬属特化药隔可能一方面对发育中的花药维管束起保护作用,另一方面又可作为酚类物质的临时贮存场所。同时研究发现忍冬属植物特化药隔异细胞群的形成及其分布特点可能具有重要的系统学意义。The specialized structure in connectives of anthers generally has important biological significance. However, information about idioblasts that accumulate phenolic compounds in connective is limited; moreover, biological significance of these phenolic idioblasts is unknown. In this study, semi-thin sectioning and cryo-sectioning techniques, and histochemical and cytochemical methods were employed to investigate morphological nature of the idioblasts in connectives of Lonicera. The experimental results showed that the idioblasts in the specialized connectives of Lonicera contained abundant phenolic compounds. In order to explore biological significance of the phenolic idioblasts in the specialized connectives, we observed ultrastructures of the phenolic idioblasts in different developmental stages, and also analyzed relationships of development of parenchyma to vascular bundles in connective of Lonicera. Our observations revealed that distribution, ultrastructure, and developmental process of the phenolic idioblasts were closely related to development of the vascular bundles in connectives, and that accumulation and degradation of phenolic compounds in these phenolic idioblasts happened alternately and regularly. So we inferred that prior to two-cell pollen stage, the specialized connective of Lonicera might be a temporary storage site of phenolic compounds to protect the developing vascular bundles from damage in anthers. By this way, the normal development of microspores and normal pollination could be ensured. However, after that stage, phenolic compounds began degrading. The degradation of these phenolic compounds might spread to other parts within the plant through vascular bundles, in order to provide precursor for synthesizing correlative components or reusing. Furthermore, our research showed that phenolic idioblasts could be a stable structural feature, and had specific distribution sites. All these f＇mdings might provide new embryological evidence for unraveling systematic relationships.

关 键 词：忍冬属 药隔 酚类物质 发育 

分 类 号：Q944[生物学—植物学]