脑缺血和NBP预处理对脑缺血沙鼠NGB和mit-Na^+,K^+-ATP酶活性的影响  

Effects of butylphthalide preconditioning on the expression of neuroglobin and the activity of mitochondrium Na^+,K^+-ATPase in cerebral ischemia of gerbils

作　　者：张镛[1] 刘振芳[1] 付庆喜[1] 张晓[1] 

机构地区：[1]山东省立医院,山东济南250021

出　　处：《山东医药》2007年第1期1-2,共2页Shandong Medical Journal

基　　金：山东省优秀中青年科学家科研基金资助项目(02BS089)

摘　　要：目的探讨丁基苯酞(NBP)对脑缺血沙鼠脑红蛋白(NGB)和线粒体N a+,K+-ATP(m it-N a+,K+-ATP)酶活性的影响。方法将蒙古沙鼠66只随机分为假手术组、脑缺血组、NBP预处理组。采用颈总动脉结扎的方法制作脑缺血模型。分别采用免疫组化染色及化学比色法检测NGB阳性细胞数和m it-N a+,K+-ATP酶活性。结果NBP预处理组NGB表达量在缺血1、10、30 m in时较脑缺血组显著增多(P<0.05);两组在缺血1、5、30、60 m in时m it-N a+,K+-ATP酶活性有统计学差异(P<0.05);NBP预处理组m it-N a+,K+-ATP酶活性与NGB表达量呈负相关(P<0.01)。结论NBP可促进脑缺血时NGB的表达,维持m it-N a+,K+-ATP酶活性。[ Objective] To investigate the effects of butylphthalide （NBP）-preconditioning on the expression of neuroglobin （NGB） and the activity of mitochondrium Na^＋,K^＋-ATPase （mit-Na^＋,K^＋-ATPase）. [.Methods] 66 Mongolian gerbils were randomly divided into 3 groups： group of sham operation, cerebral isehemia and cerebral ischemia after NBP-preconditioning. The gerbils of carotid artery occlusion was used as the research model. The expression of NGB and activity of mit-Na^＋,K^＋-ATPase were studied using immunohistochemistry and colormetric determination respectively. [Results] The amount of NGB positive cells of the NBP- preconditioning group were higher than those of the cerebral ischemia group at 1, 10, 30 rain after ischemia. The mit-Na^＋,K^＋-ATPase activity at 1, 5, 80, 60 rain after ischemia was significantly different between the cerebral ischemia group and＇ the NBP-preconditioning; group. The variations between mit-Na^＋,K^＋-ATPase activity and the amount of NGB in the NBP-preconditioning group correlated negatively （P〈0. 01）. [Conclusion] NBP can promote the expression of NGB and keen the activity of mit-Na^＋,K^＋-ATPase.

关 键 词：丁基苯酞 脑红蛋白 线粒体Na^+ K^+-ATP酵 沙土鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]