STI571诱导K562细胞耐药前后基因差异表达的研究  

Comparative gene expression profile of K562 cells resistance induced by STI571

作　　者：樊伟[1] 刘晓力[2] 张嵩[2] 杜庆锋[2] 姚锋[2] 王瑜[2] 周淑芸[2] 

机构地区：[1]济南军区青岛第二疗养院,山东青岛266071 [2]南方医科大学南方医院

出　　处：《山东医药》2007年第1期17-18,共2页Shandong Medical Journal

基　　金：国家自然科学基金(30271463);广东省自然科学基金(020086)

摘　　要：目的研究ST I571诱导K 562细胞耐药前后基因差异表达情况,探讨其耐药机制。方法用药物浓度逐步递增的方法诱导野生型K 562细胞(K 562-W)对ST I571产生耐药,用台盼蓝染色、M TT法对耐药K 562细胞(K 562-R)进行特性鉴定。应用DNA芯片技术对ST I571耐药前后的K 562细胞的基因差异表达进行检测。结果诱导出K 562-R的耐药浓度为0.5μm ol/L,活细胞比例为98.5%。芯片结果发现662个基因出现差异表达,其中335个基因表达上调,327个基因表达下调。结论利用DNA芯片技术挑选与耐药可能相关的基因,可为ST I571耐药机制的研究提供新的切入点。[Ohjective] To study the different gene expression profile of K562 cells resistance STI571 ano to investigate the potential mechanism of acquired resistance： [Methods] The wild-type K562 cells were cultured in gradually increased concentrations of STI571. Trypan blue dye and MTT assay were applied to analyses the characteristics of the resistant K562 cells （K562-R）. The gene expression difference between K562-R and K562W was examined with eDNA array. [Results] The survival percentage of K562-R was 98, 5% by Trypan blue dye and MTT assay confirmed K562-R can live steadily at 0.5μmol/L STI571. We detected different expression of 662 genes in which 335 genes were up-regulated and 327 genes were down-regulated, [Conclusion] To identified genes related to STI571 resistance with eDNA array Would provide a way or/the potential mechanism of STI571 resistance.

关 键 词：STI571 抗药性 K562细胞 基因芯片 基因表达谱 

分 类 号：R733.7[医药卫生—肿瘤]