毛细管电泳法分析蜂花粉中黄酮类化合物(英文)  

Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis

作　　者：卢挺 Edward P C Lai Jorn C C Yu 胡风祖[3] 

机构地区：[1]青海省产品质量监督检验所,青海西宁810008 [2]卡尔顿大学化学系 [3]中国科学院西北高原生物研究所,青海西宁810001

出　　处：《食品科学》2006年第12期582-587,共6页Food Science

基　　金：青海省重点科技攻关项目(2002-N-119);科技部农业科技成果转化资金项目(02EFN216301200)

摘　　要：使用毛细管电泳法(CE)检测了健康食品中常见的六种黄酮类化合物:橙皮甙、海棠甙、异鼠李素、山奈酚、槲皮素、芦丁。研究了一系列的试验参数,如:pH、缓冲溶液浓度、分离电压以及UV检测器波长等,以确定出最佳条件。使用H3BO3-Na2B4O7缓冲液(pH9.2),各分析物可在8min内分出。相对标准偏差(RSD):8次进样的迁移时间为0.77%～0.93%;峰面积为3.8%～8.6%;各检测限(S/N=3)范围为0.34μg/ml～2.9μg/ml,回收率为80.4%～113.9%。方法简单、灵敏,重现性高,线性好,无须固相萃取前处理,用于蜂花粉分析结果准确。A capillary electrophoresis （CE） method has been developed for the determination of six bioactive flavonoids that are commonly found in health foods： hesperidin, hyperin, isorhamnetin, kaempferol, quercetin and turin. The effects of several parameters, such as pH, buffer concentration, separation voltage and UV detector wavelength, were investigated to find the optimal conditions. Using a H3BO3-Nad3407 buffer （pH 9.2）, the analytes can be separated within 8 min. The relative standard deviations of migration times in eight injections were between 0.77% and 0.93%, and those of the peak areas ranged from 3.8% to 8.6%. A high reproducibility and excellent linearity was observed over two orders of magnitude, with detection limits （S/N = 3） ranging from 0.34μg ml to 2.9μg ml for all the six analytes. Recoveries ranged from 80.4 % to 113.9 %. The new method is simple, reproducible and sensitive. No solid phase extraction for sample pretreatment is necessary. Analysis results are accurate in application to bee pollens.

关 键 词：分析 黄酮类化合物 蜂花粉 毛细管电泳 

分 类 号：O623.54[理学—有机化学]