A novel strategy for in situ maskless synthesis of biochips:Self-driving micro-fluid porous type printing (SMPTP)  

作　　者：Nong Yue He Ya Fei Guo Song Li Jian-Xin Tang 

机构地区：[1]State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China [2]Key Laboratory of Green-Packaging and Application of Bionanotechnology of Hunan Province, Zhuzhou Institute of Technology, Zhuzhou 412008, China

出　　处：《Chinese Chemical Letters》2007年第1期111-114,共4页中国化学快报（英文版）

摘　　要：A novel maskless technique, self-driving micro-fluid porous type printing （SMPTP）, was reported to in situ synthesize oligonucleotide arrays on glass slide, which has the merits of low cost, high quality and simple craft. In SMPTP for fabricating gene- chips, porous fiber tubes with a number of nanometric or micron channels functioned as ＂active letters＂ and were assembled in designed patterns, which are identical to the distribution of monomers in each layer of the array, and four patterns were needed for each layer. By means of capillarity, the synthesis solution was automatically taken into porous tubes assembled in a printing plate and reached the surface. An oligonucleotide array of 160 features with four different 15-mer probes was in situ synthesized using this technique. The four specific oligonucleotide probes, including the matched and the mismatched by the fluorescent target sequence, gave obviously different hybridization fluorescent signals.A novel maskless technique, self-driving micro-fluid porous type printing （SMPTP）, was reported to in situ synthesize oligonucleotide arrays on glass slide, which has the merits of low cost, high quality and simple craft. In SMPTP for fabricating gene- chips, porous fiber tubes with a number of nanometric or micron channels functioned as ＂active letters＂ and were assembled in designed patterns, which are identical to the distribution of monomers in each layer of the array, and four patterns were needed for each layer. By means of capillarity, the synthesis solution was automatically taken into porous tubes assembled in a printing plate and reached the surface. An oligonucleotide array of 160 features with four different 15-mer probes was in situ synthesized using this technique. The four specific oligonucleotide probes, including the matched and the mismatched by the fluorescent target sequence, gave obviously different hybridization fluorescent signals.

关 键 词：TYPOGRAPHY OLIGONUCLEOTIDE In situ synthesis DNA microarrays BIOCHIPS 

分 类 号：TN495[电子电信—微电子学与固体电子学] Q78[生物学—分子生物学]