致病性鸡大肠杆菌噬菌体的分离与效价测定  

Phage Isolates and Lysis of Chicken Escherichia coli

作　　者：孙海虹[1] 马同锁[2] 于珊[1] 赵宝华[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016 [2]河北经贸大学生物科学与工程学院,河北石家庄050061

出　　处：《河北师范大学学报（自然科学版）》2007年第1期108-111,115,共5页Journal of Hebei Normal University：Natural Science

基　　金：河北师范大学科学研究基金(L2001B04)

摘　　要：鉴于临床分离的致病性大肠杆菌的耐药谱很广,常规抗生素的治疗效果较差,死疫苗往往存在毒力返祖的问题.实验从污水中分离得到了5株噬菌体,采用自行分离和鉴定的大肠杆菌为宿主菌,经纯化增殖提高效价后进行裂解试验,取得了比较满意的效果.The antimicrobial-resistant strains of Escherichia coli separated from clinic is very wide. Experiment shows that average resistant ratio of E. coli to eighteen kinds of antibacterial agents is nearly 80 percent. Common antibacterial agents＇ therapy effects are usually far from satisfaction. However, some phages obtained from sewage by purifying and propagating show better effects. The lysis ratios of 5 phages to A,B,C and D are very high.

关 键 词：鸡大肠杆菌 噬菌体 裂解 

分 类 号：S852.613[农业科学—基础兽医学]