P53、nm23蛋白和血管内皮生长因子在鼻咽癌微血管生成中的作用  

Role of P53,nm23 protein,MVD in angiogenesis of nasopharyngeal carcinoma.

作　　者：成伟[1] 梁中敏[1] 刘维奇[1] 李青[1] 梁小泉[1] 潘祖远[1] 

机构地区：[1]南方医科大学附属南海医院耳鼻咽喉科,广东佛山528200

出　　处：《临床和实验医学杂志》2007年第1期6-8,共3页Journal of Clinical and Experimental Medicine

基　　金：广东省佛山市卫生科研立项课题

摘　　要：目的探讨p53、nm23基因和血管内皮生长因子(VEGF)在鼻咽癌微血管生成及转移中的作用。方法通过免疫组化SP法对64例鼻咽癌标本中p53、nm23蛋白、VEGF及CD34抗体进行了检测。结果鼻咽癌中P53、nm23蛋白及VEGF的阳性表达率分别为65.63%、53.12%和68.75%。p53蛋白表达和VEGF有一致性,呈正相关(P<0.01)。在有淋巴结转移的肿瘤中p53蛋白和VEGF阳性表达率及微血管密度(MVD)明显高于非转移组(P<0.05)。nm23基因和VEGF在鼻咽癌标本中无一致性和直接相关性,在nm23基因表达阴性和VEGF阳性标本中MVD较高,这种现象多见于有淋巴结转移的鼻咽癌中。结论p53基因通过调控VEGF的表达影响瘤内MVD,促使鼻咽癌发生转移;而nm23基因可能不是通过调控VEGF的表达,而是通过其它途径影响鼻咽癌转移。Objective To elucidate the role of P53, nm23 and vascular endothelial growth factor （VEGF） on angiogenesis and matastasis of nasopharyngeal carcinoma. Methods Surgically resected specimens of 64 nasopharyngeal carcinomas were studied by immunohistochemical stainning for P53 protein, nm23 protein, and VEGF. Results Positive expressions of P53, nm23 protein and VEGF were found in 65.63%, 53. 12% and 68. 75 % of nasopharyngeal carcinoma specimens, respectively. There was a positive relationship between P53 and VEGF expression. The microvessel density （MVD） in p53 and VEGF positive tumors was significantly higher in patients with cervical lymph node metastases than that in patients without metastases. No correlation was identified between nm23 and MVD stainning. The MVD in nm23-negative and p53 positive tumors was significantly high. These phenomena were more easily found in patients with cervical lymph node metastases than in patients without metastases. Conclution The detection of P53, nm23 proteins may be related to the synergic expression, on angiogenesis and matastasis of nasopharyngeal carcinoma.

关 键 词：鼻咽癌 P53蛋白 NM23蛋白 血管内皮生长因子 微血管密度 血管生成 颈淋巴结转移 

分 类 号：R739.63[医药卫生—肿瘤]