CpG ODN对仔猪淋巴细胞增殖和细胞因子诱生的影响:在体和离体试验  

Effects of CpG ODN on the proliferation of lymphocytes and the induction of cytokines in piglets:in vivo and in vitro experiments

作　　者：张玲华[1] 田兴山[2] 周风珍[2] 

机构地区：[1]华南农业大学生命科学院,广东广州510642 [2]广东省农业科学院农业生物技术研究所,广东广州510640

出　　处：《中国兽医学报》2007年第1期46-50,共5页Chinese Journal of Veterinary Science

基　　金：广州市科技攻关重点资助项目(2003Z2-E0221);广东省农业攻关资助项目(2003B21403)

摘　　要：将CpG ODN、non CpG ODN分别接种初生仔猪,同时分离仔猪的外周血单核细胞,以CpG ODN、non CpG ODN对外周血单核细胞体外培养,分别检测仔猪Th1型细胞因子分泌情况。结果表明,与non CpG ODN和对照组相比,CpG ODN能在体内显著提高仔猪的淋巴细胞白介素-2诱生活性、淋巴细胞增殖、血清中IFN-γ和IL-12水平(P<0.05),亦能在体外刺激仔猪的外周血单核细胞分泌IFN-γ和IL-12(P<0.01)。这显示CpG-ODN能显著增强动物的免疫应答能力。The newborn piglets were inoculated and their separated lymphocytes were cultured with CpG ODN. non CpG ODN, respectively. In vivo experiment the proliferation of lymphoeytes, induced IL-2 activities,the content of IFN-γ and IL-12 in the serum were determined. In vitro experiments the content of IFN-γ and IL-12 in cell culture suppernatants were detected. The results showed that the above-mentioned eytokines in the newborn piglets treated with CpG ODN in vivo and in vitro were significantly higher or more than those in non CpG ODN and blank control groups.

关 键 词：CPG 免疫应答 初生仔猪 免疫增强剂 细胞因子 

分 类 号：S852.4[农业科学—基础兽医学]