重组内皮抑素抑制兔角膜新生血管的实验研究  

Role of recombinant endostatin in inhibiting corneal neovascularization induced by alkanli burn in rabbits

作　　者：袁中芳[1] 魏增涛[1] 王晓燕[2] 

机构地区：[1]济南市中心医院眼科,山东济南250013 [2]山东大学医学院免疫学研究所,山东济南250012

出　　处：《山东大学耳鼻喉眼学报》2006年第6期552-554,558,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：山东省医药卫生科研项目(2001CA2CAA1)

摘　　要：目的:探讨重组内皮抑素(Endostatin)对碱烧伤诱导的兔角膜新生血管(corneal neovasculariza-tion,CNV)的抑制作用。方法:选取新西兰大白兔18只,采用碱烧伤法制备兔CNV模型,随机分为3组,每组6只12眼。2个实验组分别以10μg/ml和50μg/ml重组内皮抑素点眼,对照组用生理盐水点眼。定期摄相,采用计算机图像分析系统比较CNV生长面积。结果:与对照组相比,2个实验组自第7 d开始CNV平均生长面积明显减少(P<0.05),经角膜组织病理学检查,新生血管明显稀少,但炎性细胞成分及浸润程度与对照组相似。结论:10、50μg/ml重组内皮抑素具有明显抑制角膜新生血管生长的作用,无毒副作用,为临床防治CNV提供了一种新的途径。Objective： To investigate the effects of recombinant human endostatin on corneal neovascularization （CNV）. Methods： Alkanli bum was used to induce neovascularization in 18 New Zealand white rabbits, which were divided into 3 groups at random, 6 in each. 10 μg/ml and 50μg/ml recombinant human endostatin were used respectively in group 1 and 2, and normal saline in group 3 （control）. The area Of CNV was determined by computer imaging analysis system. Results： The mean area of CNV of group 1 and 2 was significantly smaller than that of the control group from the 7th day（ P 〈0.05）. No significant difference was found between group 1 and 2. Conclusion： Recombinant human endostatin has prominent effect in inhibiting the corneal angiogenesis.

关 键 词：内皮押素 烧伤 化学 角膜新生血管 

分 类 号：R772.2[医药卫生—眼科]