Protective Effect of Curcumin on Endotoxin-induced Acute Lung Injury in Rats  

作　　者：连庆泉 李兴旺 尚游 姚尚龙 马利 金胜威 

机构地区：[1]Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology [2]Department of Anesthesiology, the Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical College

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第6期678-681,共4页华中科技大学学报（医学英德文版）

摘　　要：To investigate the protective effect of curcumin on endotoxin-induced acute lung injury in rats, and explore the underlying mechanisms, 24 male Wistar rats were randomly divided into 4 experimental groups： sham-vehicle （S）, sham-curcumin （C）, lipopolysaccharide （LPS）-vehicle （L）, and curcumin-lipopolysaccharide （C-L） groups. The wet/dry （W/D） weight ratio of the lung and bronchoalveolar lavage （BAL） fluid protein content were used as measures of lung injury. Neutrophil recruitment and activation were evaluated by BAL fluid cellularity and myeloperoxidase （MPO） activity in cell-free BAL and lung tissue. The levels of cytokine-induced neutrophil chemoattractant-1 （CINC-1） in lung tissues were measured by ELISA. were observed by using the HE staining. Our results the wet/dry weight ratio and protein content in BALE The histopathological changes of lung tissues showed that lung injury parameters, including were significantly higher in the L group than in the S group （P〈0.01）. In the L group, higher numbers of neutrophils and greater MPO activity in cell-free BAL and lung homogenates were observed when compared with the S group （P〈0.01）. There was a marked increase in CINC-1 levels in lung tissues in response to LPS challenge （P〈0.01, L group vs S group）. Curcumin pretreatment significantly attenuated LPS-induced changes in these indices. LPS caused extensive morphological lung damage, which was also lessened after curcumin pretreatment. All the above-mentioned parameters in the C group were not significantly different from those of the S group. It is concluded that curcumin pretreatment attenuates LPS-induced lung injury in rats. This beneficial effect of curcumin may involves, in part, inhibition of neutrophilic recruitment and activity, possibly through inhibition of lung CINC-1 expression.To investigate the protective effect of curcumin on endotoxin-induced acute lung injury in rats, and explore the underlying mechanisms, 24 male Wistar rats were randomly divided into 4 experimental groups： sham-vehicle （S）, sham-curcumin （C）, lipopolysaccharide （LPS）-vehicle （L）, and curcumin-lipopolysaccharide （C-L） groups. The wet/dry （W/D） weight ratio of the lung and bronchoalveolar lavage （BAL） fluid protein content were used as measures of lung injury. Neutrophil recruitment and activation were evaluated by BAL fluid cellularity and myeloperoxidase （MPO） activity in cell-free BAL and lung tissue. The levels of cytokine-induced neutrophil chemoattractant-1 （CINC-1） in lung tissues were measured by ELISA. were observed by using the HE staining. Our results the wet/dry weight ratio and protein content in BALE The histopathological changes of lung tissues showed that lung injury parameters, including were significantly higher in the L group than in the S group （P〈0.01）. In the L group, higher numbers of neutrophils and greater MPO activity in cell-free BAL and lung homogenates were observed when compared with the S group （P〈0.01）. There was a marked increase in CINC-1 levels in lung tissues in response to LPS challenge （P〈0.01, L group vs S group）. Curcumin pretreatment significantly attenuated LPS-induced changes in these indices. LPS caused extensive morphological lung damage, which was also lessened after curcumin pretreatment. All the above-mentioned parameters in the C group were not significantly different from those of the S group. It is concluded that curcumin pretreatment attenuates LPS-induced lung injury in rats. This beneficial effect of curcumin may involves, in part, inhibition of neutrophilic recruitment and activity, possibly through inhibition of lung CINC-1 expression.

关 键 词：acute lung injury LIPOPOLYSACCHARIDE NEUTROPHILS CINC-1 

分 类 号：R285[医药卫生—中药学]