Magnetic Iron Oxide Nanoparticles Mediated Gene Therapy for Breast Cancer-An In Vitro Study  

作　　者：韦卫中 徐春芳 吴华 

机构地区：[1]Wuhan Medical and Health Care center for Woman and Children, Tongji Medical College, Huazhong University of Science and Technology [2]Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology [3]Department of Nuclear Medicine, The First Hospital of Xiamen, Fujian Medical University

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第6期728-730,共3页华中科技大学学报（医学英德文版）

基　　金：This project was supported by a grant from the National Natural Science Foundation of China (No. 30171062).

摘　　要：The aim of this study was to evaluate the feasibility and efficacy of using TRAIL gene to treat breast cancer mediated with a novel carrier - magnetic iron oxide nanoparticles （poly- MAG-1000） coated with PEI. The magnetic iron oxide nanoparticles were used as gene carrier to transfect TRAIL gene into MCF-7 cells. The polyMAG-1000 without TRAIL gene was transfected into the tumor cells as negative control. TRAIL gene transfection with liposome as carrier served as positive control. The apoptosis of cells was detected with TUNEL method. The apoptosis ratio of tumor cells was measured with flow cytometry （FCM）. It was found that the apoptosis occurred in the tumor cells after transfection of TRAIL gene mediated by both polyMAG-1000 and liposome. The apoptosis ratio in the group with polyMAG-1000 as gene cartier was （25.11±2.85） %, whereas it was （5.06±1.05） % in the control group with polyMAG-1000 （P〈0.01）. The apoptosis ratio was as low as （18.31 ±2.44） % in the group with liposome as gene carrier （P〈0.05, as compared with the group with polyMAG- 1000 as gene carrier）. It is suggested that TRAIL gene may induce apoptosis in MCF-7 breast cancer cells. The magnetic iron oxide nanoparticles coated with PEI may be a potential gene carrier with high transfection efficacy for cancer gene therapy..The aim of this study was to evaluate the feasibility and efficacy of using TRAIL gene to treat breast cancer mediated with a novel carrier - magnetic iron oxide nanoparticles （poly- MAG-1000） coated with PEI. The magnetic iron oxide nanoparticles were used as gene carrier to transfect TRAIL gene into MCF-7 cells. The polyMAG-1000 without TRAIL gene was transfected into the tumor cells as negative control. TRAIL gene transfection with liposome as carrier served as positive control. The apoptosis of cells was detected with TUNEL method. The apoptosis ratio of tumor cells was measured with flow cytometry （FCM）. It was found that the apoptosis occurred in the tumor cells after transfection of TRAIL gene mediated by both polyMAG-1000 and liposome. The apoptosis ratio in the group with polyMAG-1000 as gene cartier was （25.11±2.85） %, whereas it was （5.06±1.05） % in the control group with polyMAG-1000 （P〈0.01）. The apoptosis ratio was as low as （18.31 ±2.44） % in the group with liposome as gene carrier （P〈0.05, as compared with the group with polyMAG- 1000 as gene carrier）. It is suggested that TRAIL gene may induce apoptosis in MCF-7 breast cancer cells. The magnetic iron oxide nanoparticles coated with PEI may be a potential gene carrier with high transfection efficacy for cancer gene therapy..

关 键 词：NANOPARTICLES TRAIL gene therapy breast cancer 

分 类 号：R737.9[医药卫生—肿瘤]