理气化痰祛瘀法对非酒精性脂肪性肝炎大鼠血浆前列环素及血栓素的影响  

Effects of Regulating Qi to Dissipate Blood Stasis and Phlegm on Plasma Prostacycline and Thromboxane in Rats with Nonalcoholic Steatohepatitis

作　　者：陈华[1] 陈芝芸[1] 严茂祥[1] 施军平[1] 何蓓晖[1] 

机构地区：[1]浙江中医药大学附属医院,浙江杭州310006

出　　处：《中华中医药学刊》2007年第1期87-89,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省教育厅资助项目(20020080)

摘　　要：目的:观察理气化痰祛瘀中药对高脂饮食诱导的非酒精性脂肪性肝炎(NASH)大鼠血浆前列环素、血栓素的影响,探讨其防治NASH的作用机理。方法:以高脂饮食喂养12周诱导W istar大鼠建立非酒精性脂肪肝模型,以不同剂量的理气化痰祛瘀中药预防及治疗,放免法测定血浆前列环素和血栓素A2的稳定代谢产物6-酮-前列环素1α(6-K-PGF1α)和TXB2含量,光镜观察肝组织切片病理学改变。结果:模型大鼠血浆TXB2含量及TXB2/6-K-PGF1α比值较相对应的正常大鼠显著升高,而6-K-PGF1α的含量与正常大鼠相比差异无显著性;应用不同剂量的理气化痰祛瘀法中药进行预防和治疗则能明显改善大鼠肝组织的脂肪变和炎症活动程度(P<0.01,P<0.05),显著降低血浆中TXB2含量和TXB2/6-K-PGF1α比值(P<0.01,P<0.05)。结论:血浆TXA2与PG I2平衡失调,可能参与大鼠NASH的发病;理气化痰祛瘀法中药能显著降低大鼠血浆TXB2水平,维持TXA2与PG I2的动态平衡,防止NASH的发生发展。Objecthe ：to investigate the Chinese Herbs with the effects of reguJating qi to dissipate blood stesis and phlegm on plasma Prostaeycline and thromboxane in rats with nonalcoholic Steatohepafitis. Methods： the model of nonalcoholic Steatohepatitis in Wistar rats were induced by a term of 12-week high fat diet, and were prevented and treated by different doses of Chinese Herbs with regulating qi to dissipate blood stasis and phlegm. , the plasma Prostacycline and thromboxane＇ s stable metabolic products-6-keto-PGF1 and TXB2 were detected with radioinmmanoassay, the pathological changes were observed with HE staining. Results. compared with the normal group, the levels of plasma Prostacycline and the ratio of TXB2/6-K-PGF1α in the model group were obviously increased, 6-K-PGF1α showed no significance with the normal group. The prevention and treatment with the Chinese Herbs could improve the steatosis of hepatocyte and inflammatory activity （P 〈 0. 01 ,P 〈 0.05） ,and remarkably decrease the amounts of plasma TXB2 and the ratio of TXB2/6-K-PGF1α （P 〈0. 01, P〈 0. 05）. Conclusion.the unbalance of TXA2 and PGI2 may participate in the onset of NASH, Chinese Hrbs with regulating qi to dissipate blood stasis and phlegm, can obviously decrease the level of plasma TXB2 of rats, keep the dynamic balance between TXA2 and PGI2, prevent the onset and development of NASH.

关 键 词：脂肪肝 非酒精性 血栓素A2 前列环素 大鼠 理气化痰祛瘀法 

分 类 号：R-33[医药卫生]