OLP中TNF-α、TGF-β_1及其受体与细胞凋亡的关系和临床意义  

Expression of TNF-α TCF-β_1 and their receptors in OLP and the correlation between TNF-α(or TCF-β_1) and apoptosis

作　　者：雷蕾[1] 钟真珍 张海伟[3] 

机构地区：[1]暨南大学医学院口腔系,广州510630 [2]东莞市东华医院口腔科 [3]暨南大学医学院病理教研室

出　　处：《现代口腔医学杂志》2007年第1期39-42,共4页Journal of Modern Stomatology

基　　金：广东省医学科研基金资助课题(A2002314)

摘　　要：目的研究肿瘤坏死因子-α(TNF-α)及其受体TNFR1,转化生长因子-β1(TGF-β1)及其受体TGFβRI在口腔扁平苔藓(OLP)的定位表达,以及与细胞凋亡的关系。方法采用免疫组化法定位检测OLP组织和正常口腔粘膜(NOM)TNF-α、TNFR1、TGF-β1、TGFβRI的蛋白表达;应用原位缺口末端标记技术,检测OLP、NOM组织细胞凋亡情况。结果TNF-α、TNFR1在OLP组织(上皮层和固有层)内表达均有增强,明显高于对照组,二者在OLP上皮内可分布于上皮细胞,TNF-α多见于巨噬细胞和淋巴细胞,TNFR1则主要分布于部分巨噬细胞。TGF-β1在OLP固有层以及TGFβRI在上皮层和固有层的表达均有增强。TGFβRI可见于OLP全层上皮细胞,在固有层,TGF-β1、TGFβRI则主要分布于成纤维细胞、血管内皮细胞和部分巨噬细胞内,二者在淋巴细胞内少见。细胞凋亡在OLP上皮层高于正常。上皮层中其阳性细胞集中分布于下部或全层上皮细胞。上皮层TNF-α表达与凋亡指数呈高度正相关。结论TNF-α、TNFR1可能是促使上皮细胞凋亡的因素之一。TNFR1、TGF-β1、TGFβRI在OLP淋巴细胞内表达缺失,可能导致OLP病程慢性迁延。Objective To investigate expression and location of death receptor pathway - tumour necrosis factor -α（ TNF -α） and its receptorl （ TNFR1 ）, the apoptotic regulater - transform growth factor -β1 （ TGF -β1 ） and its receptorl（TGFβR1）, and study the correlation between TNF-α（or TGF-β1 ） and apoptosis in oral lichen planus（OLP）. Methods Immunohistochemcial technique was used for locational detection of expression level of TNF-α, TNFR1, TGF- β1, TGFβR1, Terminal deoxynudeotidyl transferase（Tdt） - mediated deoxyuridine- 5＇ - triphosphate（dUTP） - biotin nick end - labelling （TUNEL） method was used to detect nucleosomal DNA fragmentation of apoptotic ceils in OLP and normal oral muccsa （NOM）. Results The expression of TNF- α,TNFR1 was increased in OLP. In lamina propria of OLP, it was TNF- α that was mainly seen in macrophages and lymphocytes, whereas majority TNFR1 positive cells were in macrophages and only very few in lymphocytes. Expression of TCF-β1 in OLP lamina propria and TGFβR1 in OLP tissue was increased. Both of them were chiefly localized in endotheliocytes, fibrocytes and a part of macrophages, which were absent in lymphocytes. Expression of TNF- α had a high correlation with apoptosis index in epithelium of OLP.Conclusion Death receptor pathway TNF-α/TNFR1 may be one of factors which promotes apoptosis of OLP keretinocytes, moreover, OLP with chronic course may be, in part, the result of lacking in expression of TNFR1, TCF -α and TGFβR1 in lymphocytes of OLP.

关 键 词：口腔扁平苔藓 肿瘤坏死因子 转化生长因子 受体 细胞凋亡 

分 类 号：R781.5[医药卫生—口腔医学]