肝癌患者CD4^+CD25^+调节性T细胞的检测及其临床意义  

Detection and clinical significance of CD4^+ CD25^+ regulatory T cells in patients with hepatocellular carcinoma

作　　者：杨曌[1] 牛微[1] 尚小云[1] 吴玉章[1] 赵建平[1] 王莉[1] 

机构地区：[1]第三军医大学基础部全军免疫研究所,重庆400038

出　　处：《中国癌症杂志》2007年第1期62-64,共3页China Oncology

基　　金：国家自然科学基金资助项目(No:30400437;30371329)。

摘　　要：背景与目的CD4+CD25+调节性T细胞(CD4+CD25+regulatoryTcells)在自身免疫耐受、移植和抗肿瘤应答中起着重要的作用,它们在肿瘤患者中的作用机制尚未阐明。本研究分析、探讨肝癌患者肿瘤组织和外周血CD4+CD25+调节性T细胞频率的临床意义。方法应用流式细胞仪分析肝癌患者的肿瘤组织、外周血和非肝癌对照组中单个核细胞(PBMCs)的CD4+CD25+调节性T细胞频率。结果肝癌患者外周血CD4+CD25+T细胞占PBMCs(6.9±2.8)%,肝癌组织CD4+CD25+T细胞占(6.7±1.6)%,均较对照组显著增高。并且CD4+CD25+T细胞的阳性率与淋巴结转移率和TNM分期存在相关性。淋巴结转移阳性和TNM分期越晚,CD4+CD25+T细胞的阳性率越高。结论CD4+CD25+调节性T细胞在肝癌患者中比例升高,可能是肝癌患者细胞免疫功能削弱的机制之一。Background and purpose： CD4^＋CD25^＋ regulatory T cells have played important roles in the self immunotolerance, graft and antitumor response. The research investigated the frequency of CD4^＋CD25^＋ regulatory T cells and its clinical significance in the tumor tissue and peripheral blood of the patients with hepatocellular carcinoma. Methods： Flow cytometer was used to analyze the frequency of CD4^＋CD25^＋ regulatory T cell in the tumor tissue and peripheral blood monocyte from either normal donor or the patients with hepatocellular carcinoma. Results： The frequency of CD4^＋CD25^＋ regulatory T cells in the peripheral blood of hepatocellular carcinoma patients was （6.9 ± 2.8） % , （6.7±1.6） % in the tumor tissue, both were higher than that in the control group. The levels of CD4^＋CD25^＋ regulatory T cells in tumor tissue and peripheral blood were correlated with lymphonode metastasis and TNM classification. Conclusions： The increased frequency of CD4^＋CD25^＋ regulatory T cells in the patients with hepatocellular carcinoma may he one of mechanisms that immune function was impaired in the patients.

关 键 词：肝癌 CD4 CD25 T细胞 流式细胞仪 

分 类 号：R735[医药卫生—肿瘤]