菜油烟凝聚物对大鼠肝和肺8—羟基脱氧鸟嘌呤的影响  

EFFECT OF RAPE SEED OIL CONDENSATE ON 8-OHdG LEVELS IN RAT LIVER AND LUNG

作　　者：吴一迁[1] 万曙光[1] V.L.Wilson 瞿永华[1] 

机构地区：[1]上海市肿瘤研究所

出　　处：《癌变．畸变．突变》1996年第6期342-345,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：自然科学基金

摘　　要：本文应用ＨＰＬＣ－３２Ｐ后标记技术，对经油烟凝聚物作气管灌注的大鼠肝和肺的ＤＮＡ测定８—羟基脱氧鸟嘌呤（８—ＯＨｄＧ）的水平。结果显示，经油烟凝聚物处理的大鼠肝及高剂量处理的肺８－ＯＨｄＧ的水平明显高于未经处理的大鼠，约超出２—１０倍，在分子生物学水平上，证明了油烟凝聚物对ＤＮＡ的损伤。OHdG level of rat liver and lung following intratracheal injection of rape seed oil condensate(ROC) was determined by a HPLC- 32 P-Postlabelling assay.8-OHdG was increased in liver and lung (high dose) DNA of rats in treated with ROC,and generated 2-10 fold above the control.Thus,ROC would induce the DNA lesion,8-OHdG,in rat liver and lung.

关 键 词：致癌物 羟基脱氧鸟嘌呤 油烟雾 磷32 大鼠 

分 类 号：R730.1[医药卫生—肿瘤]