纤维素酶水解棉花秸秆工艺条件的初步研究  

Study on the Technical Conditions of Hydrolyzing Cotton Straw Feed with Cellulase

作　　者：张苏江[1] 刘永兰[1] 张玲[1] 

机构地区：[1]塔里木大学动物科技学院

出　　处：《塔里木大学学报》2006年第4期1-4,共4页Journal of Tarim University

基　　金：塔里木大学校长基金资助项目(编号:200404)

摘　　要：试验采用滤纸酶活力测定法对FE-302纤维素酶的活力进行了检测,以还原糖的产量为标准,用正交设计的方法对FE-302纤维素酶水解棉秆的温度、pH值、酶用量的工艺条件进行了研究。结果显示:FE-302纤维素酶的活力是2503 U/g;温度50℃、pH值5.0、FE-302纤维素酶用量0.5%工艺条件下水解棉秸效果最好,能得到较多还原糖。FE - 302 cellulose activity was assayed by standard filter - paper - enzyme. The experiment used a 3 X 3 factorial treatment array to determine the best technical conditions of temperature, pH, and enzyme concentration of the preparations of FE - 302 cellulose toward cotton stalk. Comparing all results, it was concluded that the best condition of the preparation of the enzyme is pH 5. 0, Celsius temperature 50℃, and enzyme concentration 0.5%. Under this condition, cotton stalk hydrolyzed by the enzyme could produce much more reducing - sugar, and has the best effect.

关 键 词：棉花秸秆 FE-302纤维素酶 酶水解 

分 类 号：Q55[生物学—生物化学]