分化抑制因子Id2和Id3在肿瘤细胞中的表达  

Expression of inhibitors Id2 and Id3 of differentiation mRNA in tumor cells

作　　者：汪萍[1] 李晓军[1] 贾丽[1] 马百坤[1] 黄宇烽[1] 

机构地区：[1]南京军区南京总医院解放军检验医学研究所,江苏南京210002

出　　处：《医学研究生学报》2007年第1期20-23,共4页Journal of Medical Postgraduates

基　　金：江苏省六大人才高峰基金课题资助项目(批准号:050203D)

摘　　要：目的:研究分化抑制因子(Id)基因家族在不同肿瘤细胞及健康人外周单个核细胞(PBMC)中的表达情况,为探讨Id基因与肿瘤生长的关系提供实验依据。方法:提取血液病患者和健康人PBMC、人Burk itt’s B淋巴瘤细胞(Daud i)、人T淋巴细胞性白血病细胞(Jurkat)、人慢性髓性白血病细胞(K562)、人组织细胞性淋巴瘤细胞(U937)、人前列腺癌细胞(PC-3)、人胃癌细胞(SGC)、人肺癌细胞(SPC),人卵巢癌细胞(COC2)的细胞总RNA,根据Id2和Id3 cDNA全长序列合成两对引物,运用RT-PCR方法,对以上细胞中Id2和Id3 mRNA的表达进行半定量分析。结果:不同的Id基因在不同人肿瘤细胞中的表达具有一定差异性:Id2在健康人PBMC和各类肿瘤细胞中均呈普遍表达趋势;Id3在健康人淋巴细胞中未见表达或表达水平极弱,而在不同肿瘤细胞中的表达有差异,Id3在PC-3中的表达最高,K562中最低。不同的Id基因在同种细胞中的表达的差异性也较大,Id2 mRNA的表达水平显著高于Id3mRNA的表达水平。Id2、Id3mRNA在血液病患者PBMC中均有不同程度的的表达。结论:不同的Id基因在不同的肿瘤细胞中有不同的表达水平,Id3的异常表达在肿瘤细胞增殖及肿瘤的发生中可能发挥一定的作用。Objective：To study the expression of Id mRNA in human malignant tumor cells and the peripheral blood mononuclear cell （ PBMC ） of the healthy adaults. Methods ： The total cellular RNA was extracted from the peripheral blood mononuclear cell（PBMC） of the patients with hematologic disorders, PBMC from healthy adults and eight tumor cell lines （Daudi, Jurkat, K562, U937, PC-3, SGC. SPC-A1, and COC2）. The primers based on the reported sequences of Id2, and Id3 were synthesized. The mRNA expression levels of Id2 and Id3 in the PBMC and tumor cells were detected by RT-PCR techniques. Results：Id2 and Id3 mRNA have different expression levels in the different cells. Id2 is widely expressed in many cell types, both in normal and malignant cells. The levels of Id3 mRNA expression levels in tumor cells and PBMC from hematologic disorder patients are significantly higher than that in healthy adult＇ s PBMC. Conclusion ： Id2 and Id3 have different expression patterns in different cell types. It seems that Id3 may play some important roles in the proliferation and differentiation of tumor cells, as well as in the development and growth of tumors.

关 键 词：分化抑制因子 逆转录聚合酶链反应 血液病 肿瘤细胞 

分 类 号：R730.23[医药卫生—肿瘤]