K562细胞多重耐药机制的实验研究  

Study on the mechanism of multidrug resistance in K562 cell

作　　者：杨冬艳[1] 康劲松[2] 孔晓霞[2] 张宏宇[2] 高凤彤[1] 王辉[1] 

机构地区：[1]吉林大学中日联谊医院,吉林长春130033 [2]吉林大学基础医学院病理生理学教研室

出　　处：《中国实验诊断学》2007年第1期29-31,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨K562细胞多重耐药性形成的分子机制。方法采用MTT法分别测定K562及阿霉素耐药的K562细胞对顺铂的耐药程度,采用RT-PCR检测细胞表面MDR、MRP、GST及MGMT分子表达水平,并确定其与耐药性的关系。结果顺铂对K562细胞的抑制率明显高于阿霉素耐药的K562细胞,同时阿霉素耐药的K562细胞的表面MDR、MRP、GST及MGMT的表达明显增高。结论MDR、MRP、GST及MGMT的高表达是K562细胞形成多重耐药性的重要机制之一。Objective To explore the mechanism of muhidrug resistance in K562 cell. Methods MTT colorimetric analysis was used to measure the inhibitory effect of cisplatin（DDP） on K562 cell and K562/ADM cell and the resistance index. RT-PCR was used to evaluate the mRNA expression of MDR1 ,MRP~ GST and GMST genes. Results The inhibition rate of cisplatin（DDP） on K562 cell is higher than that of K562/ADM cell. Simultaneously the mRNA expression d MDR1 ,MRP、GST and GMST in K562/ADM cell is higher that of K.562 cell. Conclusion The higher mRNA expression of MDR1 ,MRP、 GST and GMST genes is the main causes of multidrug resistance in K562/ADM.

关 键 词：K562细胞 多重耐药 甲基鸟嘌呤甲基转移酶 谷胱苷肽S-转移酶 多药耐药相关蛋白 

分 类 号：R733.7[医药卫生—肿瘤]