不同膳食脂肪酸构成对乳腺癌大鼠BRCA1表达的影响  

Effect of different ratio of n-6/n-3 polyunsaturated fatty acid on expression of BRCA1 in the MNU-induced breast cancer rats

作　　者：王静[1] 糜漫天[1] 韦娜[1] 王斌[1] 

机构地区：[1]第三军医大学军事预防医学院营养与食品卫生学教研室,重庆400038

出　　处：《实用医药杂志》2007年第1期84-86,共3页Practical Journal of Medicine & Pharmacy

基　　金：国家自然科学基金资助项目(30500408)

摘　　要：目的探讨不同比例n-6/n-3多不饱和脂肪酸(PUFA)对甲基亚硝基脲(MNU)诱导的乳腺癌大鼠乳腺癌易感基因(BRCA1)表达的影响。方法雌性SD大鼠65只随机分为5组,即n-6PUFA组、10:1n-6/n-3组、5:1n-6/n-3组、1:1n-6/n-3组和正常对照组,前四组以50mg/kg体重MNU单次腹腔注射诱导大鼠乳腺肿瘤发生,正常对照组注射等体积无菌生理盐水。给药后立即分组喂养不同饲料,并在8周和18周时处死动物剪取乳腺组织,利用RT-PCR和Westernblot技术检测组织中BRCA1mRNA和蛋白表达状况。结果四组乳腺癌大鼠BRCA1表达较正常对照组均有所下降,四组中BRCA1的表达以1:1n-6/n-3组最强,并随n-6/n-3比值增大而下降。结论不同膳食脂肪酸构成对MNU诱导的乳腺癌大鼠BRCA1的表达具有不同影响,1:1n-6/n-3膳食脂肪酸构成能有效抑制乳腺癌大鼠BRCA1表达的下降。Objective To observe the effect of different ratio of n-6/n-3 polyunsaturated fatty acid on expression of BRCA1 in the MNU-induced breast cancer rats.Methods 65 female SD rats were randomly divided into 5 groups： n-6, 10：1 n-6/n-3, 5：1 n-6/n-3,1：1 n-6/n-3 and normal controlled group. Except controlled group received physiological saline, all rats were given a single ip injection of MNU （50mg/kg BW） and switched to different diets containing fatty acids mentioned above. After 8w and 18w, the expressions of BRCA1 in the breast tissue of different group were determined by RT-PCR and Western blot.Results The expressions of BRCA1 in breast cancer rats were lower than normal control, they were highest in the 1：1 n-6/n-3 group and gradually decreased with the ratio of n-6/n-3 increasing. Conclusion Different dietary fatty acid compositions can diversely influence the expression of BRCA1 in MNU-induced breast cancer rats; 1：1 n-6/n-3 can significantly inhibit the decrease of BRCA1 expression in breast cancer rats.

关 键 词：N-6/N-3多不饱和脂肪酸 乳腺癌大鼠 BRCA1 

分 类 号：R151.2[医药卫生—营养与食品卫生学] R737.9[医药卫生—公共卫生与预防医学]