脊髓损伤后通过P38丝裂素活化蛋白激酶途径上调胶质纤维酸性蛋白的表达  

The up-regulation of glial fibrillary acidic protein media through P38-mitogen activated protein kinase pathway after spinal cord injury

作　　者：于春雷[1] 王翀昊[2] 张弘[1] 刘长江[1] 腾宇飞[3] 

机构地区：[1]吉林大学第四医院神经外科,吉林130011 [2]吉林大学第一医院神经外科 [3]吉林大学第四医院骨外科,吉林130011

出　　处：《脊柱外科杂志》2006年第6期359-362,共4页Journal of Spinal Surgery

摘　　要：目的探讨脊髓损伤(SCI)后受损的脊髓组织与其周围组织中胶质纤维酸性蛋白(glialfibrillaryacidicpro-tein,GFAP)和P38丝裂素活化蛋白激酶(P38-mitogenactivatedproteinkinase,P38MAPK)的表达情况。方法将80只大鼠随机分成10组:正常对照组、损伤后1d组、损伤后4d组、损伤后7d组、损伤后14d组、损伤后应用SB203580药物1d组、4d组、7d组、14d组及健康大鼠给药组,每组均8只。应用westerblot技术检测各组损伤组织及损伤周围组织的P38MAPK及GFAP的表达。结果受损脊髓组织从伤后第1dP38MAPK、GFAP开始上升,持续到第7d开始回落,第7d是表达最高峰。损伤组织P38MAPK及GFAP的表达具有明显正相关性(r=0.854,P<0.05),损伤周围组织P38MAPK及GFAP的表达无明显相关性(r=0.554,P>0.05)。结论损伤组织GFAP表达上调是通过P38MAPK介导的,损伤周围组织GFAP上调与P38MAPK表达无关。Objective To investigate the expression of glial fibrillary acidic protein （GFAP） and P38-mitogen activated protein kinase （P38MAPK） in the injury tissue after spinal cord injury. Methods 80 rats were divided into 10 groups randomly： control group, Group 1d, 4d, 7d、14d after injury; Group 1d, 4d, 7d, 14d after supplying SB203580 and group of healthy rats given medicine group. Each group had 8 rats. The expression of GFAP and P38MAPK were detected with western-blot method. Results The expression of P38MAPK and GFAP began to increase at 1d after injury, decrease at 7d after injury. The expression of P38MAPK showed obviously positive correlation with the GFAP in injury tissue （r = 0.854, P 〈 0.05）, the expression of P38MAPK showed no correlation with the GFAP around injury tissue （r = 0.554, P 〉 0.05）. Conclusion The P38MAPK media the increasing of GFAP on injury tissue, the up-regulation of GFAP has no relationship with P38MAPK around the injury tissue.

关 键 词：脊髓损伤 瘢痕 星形细胞 神经胶质原纤维酸性蛋白质 

分 类 号：R651.2[医药卫生—外科学]