腺病毒介导的人骨形成蛋白2基因转染促进下颌牵张成骨的实验研究  

Effect of bone morphogenetic proteins-2 on mandibular distraction osteogenesis in rabbits

作　　者：陈安威[1] 魏奉才[1] 王克涛[1] 孙善珍[2] 刘少华[1] 

机构地区：[1]山东大学齐鲁医院口腔颌面外科,济南250012 [2]山东大学口腔医学院病理科

出　　处：《中华口腔医学杂志》2007年第1期15-17,共3页Chinese Journal of Stomatology

基　　金：国家自然科学基金(30471796)

摘　　要：目的探讨局部应用腺病毒介导的人骨形成蛋白2(adenovirus vectors containinghuman bone morphogenetic proteins 2,Ad-hBMP-2)对兔下颌骨牵张成骨的影响。方法 24只新西兰大白兔随机分为实验组(12只)和对照组(12只),并建立下颌骨双侧牵张成骨模型。经过5 d 潜伏期后,以0.5 mm/12 h 的速度牵张7 d。固定期第1天,在实验组骨牵张区注射0.2 ml 滴度为10^(12)pfu/L的 Ad-hBMP2,对照组骨牵张区注射0.2 ml 滴度为10^(12)pfu/L 的腺病毒介导的增强型绿色荧光蛋白。在固定期第7、14、28天对下颌骨牵张区进行骨密度及新生骨量比较。结果 Ad-hBMP-2治疗组牵张区骨密度及新生骨量明显高于对照组。结论腺病毒介导的人骨形成蛋白2具有促进兔下颌牵张成骨的作用。Objective To investigate the Effect of recombinant adenovirus vectors containing human Bone morpbogenetic proteins-2 （Ad-hBMP-2） on the for mandibular distraction osteogenesis （DO） in rabbits. Methods Twenty-four New Zealand white rabbits were randomly divided into experimental group, and control group and underwent mandibular distraction osteogenesis. After 5 days latency, the distracters were activated at a speed of 0. S mm every 12 hours for 7 days, then on the first day in the consolidation period, the distraction gaps of experimental group were injected with 0. 2 ml Ad-hBMP2 10^12pfu/L, while the animals of control group were injected with 0. 2 ml Ad-EGFP 10^12 pfu/L . At the 7 th and 28 th day of consolidation period, specimens were obtained, X-ray and histomorphology were performed. The bone density and the quantity of new bone formation in the distraction gaps were observed and compared between the two groups at different consolidation period. Results Ad-hBMP-2 treated specimens demonstrated an increased amount of new bone formation Conclusions Adenovirally-mediated delivery of BMP-2 can locally increase bone deposition during DO, which may potentially shorten the consolidation period.

关 键 词：骨形成蛋白2 基因疗法 牵张成骨 

分 类 号：R782[医药卫生—口腔医学]