脂多糖刺激牙龈成纤维细胞产生白细胞介素11、6的研究  

Production of interleukin-11 and interleukin-6 in cultured human gingival fibroblasts with the stimulation of lipopolysacchrides

作　　者：和璐[1] 长泽敏行[2] 石川烈[2] 

机构地区：[1]北京大学口腔医学院.口腔医院牙周科,100081 [2]东京医科齿科大学齿周病分野

出　　处：《中华口腔医学杂志》2007年第1期34-36,共3页Chinese Journal of Stomatology

摘　　要：目的观察牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)、伴放线放线杆菌(Actinobacillus actinomycetemcomitans,Aa)、大肠杆菌(Escherichia coli,Ec)的脂多糖(lipopolysacchrides,LPS)刺激人牙龈成纤维细胞(human gingival fibroblasts,HGF)合成白细胞介素(interleukin,IL)-11和IL-6的能力,并了解内源性前列腺素是否介导 IL-11和 IL-6的产生。方法将3种浓度的 LPS(0.1、1、10 mg/L)分别作用于体外培养的 HGF 24 h,另外在10 mg/L 的 LPS 中加入10^(-6)mol/L 的吲哚美辛后再分别刺激 HGF 24 h,ELISA 法检测 HGF 上清液中 IL-11和 IL-6含量的变化。结果 Aa-,Ec-LPS(10 mg/L)和 Pg-LPS(1、10 mg/L)刺激 HGF 后 IL-11水平显著提高;Pg-,Ec-LPS(0.1、1、10 mg/L)和Aa-LPS(1、10 mg/L)刺激 HGF 产生 IL-6的水平明显增高;这些作用均受到吲哚美辛的抑制作用。结论 LPS 使 HGF 产生 IL-11和 IL-6的水平明显增加,并受到内源性前列腺素的正向调控。Objective To observe the effects of Porphyromonas gingivalis （Pg）, Actinobacillus （Aa） ,Escherichia coli（Ec） lipopolysacchrides（LPS） on the production of IL-11 and IL-6 from healthy human gingival fibroblasts（HGF） , and the effects of endogenous prostaglandin on HGF IL-11 and IL-6 production stimulated with the above LPS. Methods HGF were stimulated with Pg-, Aa-, Ec- LPS of different concentrations （0. 1,1,10 mg/L ） for 24 h. And HGF were also stimulated with the combinations of 10 mg/L Pg-, Aa-, Ec-LPS and 10^-6 mol/L indomethacin respectively for 24 h. Levels of IL- 11 and IL-6 in the supernatants were quantitated by ELISA. Results LPS from Aa, Ec, at the concentration of 10 mg/L and from Pg at the concentrations 1, 10 mg/L significantly augmented IL-11 production by HGF. IL-6 production was also significantly increased by stimulation with Aa-LPS at concentrations 1, 10 mg/L and with Ec-,Pg-LPS at concentrations 0. 1,1,10 mg/L. In addition, IL-11 production was lower than IL-6 production by HGF stimulated with LPS. Indomethacin significantly inhibited IL-6 and IL-11 production in LPS-stimulated HGF. Conclusions Aa-, Pg-, Ec-LPS may significantly increase IL-11 and IL-6 level in the supernatants of HGF, and endogenous prostaglandin may upregulate IL-11 and IL-6 production in LPS-stimulated HGF.

关 键 词：脂多糖类 白细胞介素11 白细胞介素6 紫单胞菌 龈 

分 类 号：R78[医药卫生—口腔医学]