吸烟对大鼠肺内谷胱甘肽与γ-GCS的影响及乙酰半胱氨酸的干预作用  

Effect of smoking on GSH and γ-GCS in lungs of rats and the protective role of N-acetylcysteine

作　　者：邹朝霞[1] 何志义[1] 冉丕鑫[1] 

机构地区：[1]广州医学院第一附属医院广州呼吸疾病研究所

出　　处：《中国病理生理杂志》2007年第1期86-89,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30170401);广东省自然科学基金资助项目(No.000321)

摘　　要：目的:探讨被动吸烟对SD大鼠BALF中谷胱甘肽浓度(GSH)和肺组织中γ-GCS表达的影响以及乙酰半胱氨酸NAC的干预作用。方法:SD大鼠被分成吸烟、吸烟同时加用NAC和单纯吸烟3组,分别采用GSH特异性的DTNB-GSH还原酶循环法及免疫组化的方法检测GSH浓度及γ-GCS的表达水平。结果:单纯被动吸烟大鼠肺BLAF中GSH水平较对照和加用NAC组明显升高(P<0.05)。肺组织内γ-GCS阳性染色明显增强;吸烟与吸烟同时服用NAC组比较没有显著区别(P>0.05)。结论:被动吸烟促进SD大鼠肺内GSH和γ-GCS表达,这可能是机体的一种自我代偿反应;NAC对吸烟导致的气道炎症具有一定的保护作用。AIM： To investigate the effects d cigarette smoking on the GSH and γ-GCS in SD rats, and the protective role of N - acetylcysteine （NAC）. METHODS： SD rats were exposed to cigarette smoke, and GSH eoncentralions in BALF were measured by GSH -specific DTNB -GSH reductase recycling assay. Expression of γ -GCS protein in lung tissue was determined by immunohistochemistry. RESULTS： Exposure to cigarette smoking increased GSH content in BALF and the expression of γ -GCS protein in the lung tissue of SD rats. CONCLUSION： Cigarette smoking affects intracellular GSH eoncentration and the expression of γ-GCS protein in lungs d SD rats. NAC attenuates the inflammatory reaction resulting from cigarette smoking.

关 键 词：吸烟 大鼠 谷胱甘肽 谷氨酰半胱氨酸合酶 

分 类 号：R363[医药卫生—病理学]