雷公藤甲素抑制PBMC分泌IL-1β蛋白量与IL-1β基因多态性有关(英文)  

Inhibitory effect of triptolide on production of IL-1β from PBMC is associated with IL-1β gene polymorphism

作　　者：盛冬云[1] 涂胜豪[1] 陈红波[1] 胡永红[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所,湖北武汉430030

出　　处：《中国病理生理杂志》2007年第1期90-94,共5页Chinese Journal of Pathophysiology

基　　金：Supported by National Natural Science Foundation of China(No.90209049)

摘　　要：目的:探讨雷公藤甲素对外周血单个核细胞(PBMC)分泌白细胞介素-1β(IL-1β)的抑制作用与IL-1β基因多态性的关系。方法:采用PCR-RFLP法对31名健康志愿者IL-1β基因启动子区-511位点C-T多态性进行基因型检测,同时进行PBMC培养,用脂多糖(LPS)刺激培养细胞,并予以雷公藤甲素处理PBMC,收集培养上清液,ELISA法检测上清液中IL-1β的含量。结果:携带IL-1β-511T/T纯合子基因型PBMC经LPS刺激后IL-1β的分泌量明显较非T/T纯合子为高(P<0.05);雷公藤甲素能显著抑制LPS诱导的C/C和C/T基因型PB-MC分泌IL-1β(P<0.05),但对T/T基因型的抑制作用不明显(P>0.05)。结论:IL-1β基因-511C-T多态性与IL-1β的分泌量相关,雷公藤甲素对不同基因型的IL-1β抑制作用有差异,这可能是导致雷公藤药理作用出现个体差异的原因之一。AIM： To explore whether the inhibitory effect of triptolide on IL - 1β production by PBMC is associated with IL - 1β gene polymorphisms. METHODS： IL - 1β gene polymorphism was analyzed in 31 healthy volunteers. From genomic DNA, the C -T polymorphism at IL - 1β -511 was typed by PCR - RFLP. Meanwhile the IL - 1β was also measured in the supernatants of the cultured and stimulated peripheral blood mononuclear cells （PBMC） by ELISA. RESULTS： After LPS stimulation in PBMC cultures of healthy subjects, the secretion levels of IL - 1β in 9 volunteers who carried IL - 1β - 511 T/T genotype were higher than in volunteers who are not T/T genotype （ P 〈 0. 05 ）. Triptolide suppressed the production of IL - 1β significantly in LPS - treated hnman PBMC carried C/C and C/T genotype （ P 〈 0. 05 ）, but this signitleant inhibitory effect of triptolide was not seen in T/T genotype （ P 〉 0. 05 ）. CONCLUSION： The gene polymorphism at IL- 1β -511 was related to the production oflL - 1β, and the inhibitory effect of triptolide on the production of IL - 1β was different in C/C, C/T, T/T genotype of IL - 1β -511, which may be one of the reasons for the phenomenon that people respond differently to triptolide.

关 键 词：白细胞介素1 基因 多态现象(遗传学) 雷公藤属 关节炎 类风湿 

分 类 号：R363[医药卫生—病理学]