SR-AⅡ和CD36的表达水平与AGEs及糖尿病并发症的相关性研究  

Expression of SR-A II and CD36 in white blood cells correlate with plasma AGEs levels and diabetic complications

作　　者：陈万群[1] 骆婕[2] 彭锐锐[2] 吴颜晖[1] 莫宏波[1] 冯烈 李菊香 刘誉[1] 

机构地区：[1]暨南大学医学院生化教研室 [2]暨南大学医学院临床医学系 [3]暨南大学医学院附属华侨医院,广东广州510632

出　　处：《中国病理生理杂志》2007年第1期168-172,共5页Chinese Journal of Pathophysiology

基　　金：暨南大学"科技创新"资助项目(No.C04074);暨南大学引进人才基金资助项目(No.51204004)

摘　　要：目的:分析糖尿病患者白细胞中清道夫受体SR-AⅡ和CD36的表达水平与血浆晚期糖基化终产物(AGEs)浓度以及糖尿病并发症之间的相关性。方法:收集具有合并症的糖尿病患者抗凝血78例,用荧光分光光度法测定血浆AGEs浓度。用Trizol法提取白细胞RNA,再以RT-PCR法测定SR-AⅡ和CD36的相对表达水平。结果:在测定的糖尿病各合并症中,SR-AⅡ在糖尿病性肾病中表达水平最高,在脂肪肝患者中最低;CD36在脂肪肝患者中最高,在冠心病患者中最低。在高血压患者中这2种受体的表达水平均比较高,但在白内障患者中2种受体的表达相对较低。血浆AGEs浓度与白细胞中CD36表达水平呈负相关(r=-0.89,P<0.01),但与SR-AⅡ的表达为正相关(r=0.82,P<0.05)。结论:血浆高浓度AGEs刺激白细胞中SR-AⅡ表达增加,糖尿病性肾病和脂肪肝分别与SR-AⅡ、CD36的表达增加有密切关系,部分糖尿病患者CD36的表达较低可能是导致他们血浆AGEs水平过高的原因之一。AIM. To investigate the correlation of the expression of scavenger receptors SR - AⅡ and CD36 in white blood cells （WBCs） with the plasma levels of advanced glycosylation end products （AGEs） and different diabetic complications. METHODS： Blood samples were collected from 78 patients with diabetic complications. The levels of plasma AGEs were determined by using spectrofluorimetry. RNA in WBCs was extracted with Trizol reagent and the mRNA levels of SR - A H and CD36 were determined by RT - PCR. RESULTS： In the patients tested, the mRNA level of SR - AⅡ was found to be the highest in those with diabetic nephropathy, and. lowest in those with fatty liver. The expression of CD36 was found to be the highest in diabetic patients with fatty liver and lowest in those with coronary heart disease. The expression of. beth receptors in WBCs showed significantly higher levels in diabetic patients with hypertension, and lower in those with cataract. The plasma levels of AGEs negatively correlated with mRNA levels of CD36 （ r = -0. 89 ,P 〈0. 01 ）, while positively correlated with SR - A H mRNA levels （r = - 0. 82, P 〈 0. 05）. CONCLUSION： The increased plasma levels of AGEs may stimulate the expression of SR - A H in WBCs, and higher expression of SR - AⅡ and CD36 was significantly related to diabetic complications, nephropathy and fatty liver, respectively. However, low expression of CD36 in some diabetic patients with complications might be important causes for their high plasma AGEs levels.

关 键 词：CD36 糖尿病 糖基化终产物 高级 受体 清道夫 

分 类 号：R363[医药卫生—病理学]