高效液相色谱法测定邻烯丙基苯酚在草莓果实中的消解动态和残留  

Studies on Degradation and Final Residue of the Bionic Fungicide o-Allylphenol in Strawberry Fruit by High Performance Liquid Chromatography

作　　者：夏源源[1] 孟昭礼[2] 李健强[1] 张文华[1] 侯红利[3] 李永红[3] 周向阳[3] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094 [2]莱阳农学院植保系,山东266109 [3]深圳市农作物良种引进中心,深圳518040

出　　处：《分析化学》2007年第1期79-82,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No3071160);深圳市科技与信息局计划项目(NoS040161);教育部"新世纪优秀人才支持计划"(NoNCET-04-0128)资助项目

摘　　要：采用高效液相色谱法定量分析仿生杀菌剂邻烯丙基苯酚在草莓果实中的消解动态和最终残留。结果表明，施药处理的草莓果实的果浆用丙酮提取，经石油醚萃取、浓缩和定容后采用高效液相色谱分析（HPLC）方法，在273nm波长检测条件下，标准曲线方程为Y：10．049×-44．395，（R^2=0．997）。经测定，邻烯丙基苯酚的最低检出量为4．2ng，在草莓中的最低检出浓度是0．02mg／kg。本方法的添加回收率为79．7％-84．6％，相对标准偏差1．78％-4．01％。测定邻烯丙基苯酚在草莓中的消解动态及最终残留显示，药剂在草莓中的半衰期为4．40d，草莓收获时药剂的消解率在90％以上。The degradation and residue of bionic fungicide o-allylphenol in the strawberry fruit were studied by means of high performance liquid chromatography （HPLC）. The residue of o-allylphenol was extracted from strawberry with acetone, purified by liquid-liquid partition, concentrated to a small volume, and then determined by HPLC with UV detection at 273 nm, The equation of the calibration curves was Y = 1004.9X - 4439.5, （R^2 = 0. 997）. The minimum detectable amount of o-allylphenol was 4.2 ng, the minimum detectable concentration of o-allylphenol in the sample of strawberry was 0. 02 mg/kg. The average recoveries and relative standard deviations of the method were 79.7% -84.6% and 1.8% -4.0% respectively. The degradation and final residue in strawberry were determined with the method. The results indicated that the halflife of o-allylphenol in strawberry was 4.4 days, and o-allylphenol in strawberry fruit was degraded more than 90% before harvesting.

关 键 词：高效液相色谱 仿生杀菌剂 邻烯丙基苯酚 残留分析 消解动态 草莓 

分 类 号：TS207.53[轻工技术与工程—食品科学]