5种生物胺的毛细管胶束电动色谱分离  

Separation of Biogenic Amines by Micellar Electrokinetic Chromatography

作　　者：乔成栋[1] 宋平顺[2] 严祥[3] 蒋生祥[1] 

机构地区：[1]中国科学院兰州化学物理研究所 [2]甘肃省药品检验所,兰州730000 [3]兰州大学第一医院,兰州730000

出　　处：《分析化学》2007年第1期95-98,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助(No20375045)

摘　　要：在胶束电动色谱模式下考察了影响生物胺分离的几个因素(如缓冲溶液及SDS浓度、pH、表面活性剂、分离温度及外加电压等)并优化了分离条件,建立了一种简单快速的利用毛细管胶束电动色谱DAD检测器分离分析生物胺的新方法。当缓冲溶液为40mmol/L硼酸缓冲液(pH8.5)、68mmol/LSDS,分离电压为25kV、分离温度为25℃时,5种生物胺在7min内实现了令人满意的基线分离。在优化的条件下,对其线性范围、检出限和重现性进行了测定。结果表明,生物胺的迁移时间的重现性<0.8%,面积的重现性<4%。A novel, simple and rapid method for the separation of biogenic amines by micellar electrokinetic chromatography with diode array detection was developed. The effect of some key factors, such as concentration of buffer and sodium dodecyl sulfate （SDS）, pH, surfactant, capillary temperature and applied voltage on the separation of biogenic amines was investigated. Under the optimized conditions, the baseline separation of five biogenic amines was achieved with satisfactory repeatability and high separation efficiency in 7 rain using 40 mmol/L borate buffer （pH 8.5） containing 68 mmol/L SDS , under 25 kV applied voltage at 25℃. The linear range, the limit of detection and the repeatability were also investigated under the optimized conditions. The RSD （n =6） of the migration time is 〈0.8% and the RSD （n =6） of peak area is less than 4% for each biogenic amines, the linear ranges are wide and the limit of detection is low.

关 键 词：胶束电动色谱 分离 生物胺 5-甲氧基色胺 5-羟基色氨酸 

分 类 号：R341[医药卫生—基础医学]