Structural and Functional Changes of Immune System in Aging Mouse Induced by D-Galactose  

作　　者：HONG-BIN DENG CHUN-LEI CHENG DA-PENG CUI DIAN-DONG LI LI CUI NIAN-SHENG CAI 

机构地区：[1]Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China [2]Preclinical Medicine College, Jilin University, Changchun 130021, Jilin, China

出　　处：《Biomedical and Environmental Sciences》2006年第6期432-438,共7页生物医学与环境科学（英文版）

基　　金：This work was supported by a grant from the Major State Basic Research Development Program Foundation of China (No. 2007CB507406) and a grant from the National Natural Science Foundation of China (No. 30600659).

摘　　要：Objective To investigate the role of D-galactose, especially in the structural and functional changes of the immune system in aging. Methods Serum levels of advanced glycation end-products （AGE） were determined by ELISA method. Ultra-structures of thymus and spleen were detected by transmission electron microscopy. MTT method was used to determine the lymphocyte proliferation. IL-2 activity was determined by bioassay. Northern blot was used to detect the IL-2 mRNA levels. Results Serum AGE levels of D-galactose- （P〈0.01） and AGE-treated （P〈0.05） mice （n=8） were increased significantly. The ultra-structures of thymus and spleen in D-galactose- and AGE-treated mice showed regressive changes similar to those in the aged control group. The lymphocyte mitogenesis and IL-2 activity of spleen were also decreased significantly （P〈0.01, n=8）. The change of IL-2 activity shown by Northern blot resulted from the change of mRNA expression. The AGE plus aminoguanidine group, however, showed no significant change in these parameters in comparison with the young control group （P〈0.01 or P〈0.05, n=8）. Conclusion D-galactose and AGE lead to a mimic regression change of aging in the immune system in vivo.Objective To investigate the role of D-galactose, especially in the structural and functional changes of the immune system in aging. Methods Serum levels of advanced glycation end-products （AGE） were determined by ELISA method. Ultra-structures of thymus and spleen were detected by transmission electron microscopy. MTT method was used to determine the lymphocyte proliferation. IL-2 activity was determined by bioassay. Northern blot was used to detect the IL-2 mRNA levels. Results Serum AGE levels of D-galactose- （P〈0.01） and AGE-treated （P〈0.05） mice （n=8） were increased significantly. The ultra-structures of thymus and spleen in D-galactose- and AGE-treated mice showed regressive changes similar to those in the aged control group. The lymphocyte mitogenesis and IL-2 activity of spleen were also decreased significantly （P〈0.01, n=8）. The change of IL-2 activity shown by Northern blot resulted from the change of mRNA expression. The AGE plus aminoguanidine group, however, showed no significant change in these parameters in comparison with the young control group （P〈0.01 or P〈0.05, n=8）. Conclusion D-galactose and AGE lead to a mimic regression change of aging in the immune system in vivo.

关 键 词：D-GALACTOSE Aging-mimetic model Advanced glycation end-products ULTRA-STRUCTURE Nonenzyme glycation AMINOGUANIDINE 

分 类 号：R392[医药卫生—免疫学]