荣昌猪白细胞介素-2和白细胞介素-4基因的克隆与序列分析  

Cloning and sequence analyzing of interleukin-2 and interleukin-4 cDMA from the lymphocyte of Rongchang Pig

作　　者：张芳 郭万柱[1] 吴华[1] 

机构地区：[1]四川农业大学动物生物技术中心,四川雅安625014

出　　处：《黑龙江畜牧兽医》2007年第1期10-12,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家"973"项目(2004CCA01800)

摘　　要：将荣昌猪外周血淋巴细胞在刀豆素A（ConA）的刺激下培养24—70h后，提取总RNA，应用RT—PCR技术扩增白细胞介素-2（IL-2）和白细胞介素-4（IL-4）cDNA，克隆到pMD18-T载体并测序。结果表明：克隆的IL-2cDNA全长为522个碱基，ORF为465个碱基，编码154个氨基酸。与GenBank公布的猪IL-2比对同源性均为99．8％；克隆的IL-4cDNA全长411个碱基，ORF为402个碱基，编码133个氨基酸，与GenBank公布的猪IL-4比对同源性均在98．0％以上，从而证实成功克隆了荣昌猪IL-2和IL-4基因cDNA。The interleukin-2 and interleukin-4 cDNA of Rongchang Pig were amplified by RT PCR from total RNA extracted from blood lymphocytes, which were cultivated and stimulated with 7 μg/mL ConA in vitro for 24 -72 hours. The amplified cDNA was cloned into PMD18 - T vector. The nucletide acid sequences of cloned Rongchang Pig interleukin -2 and interleukin -4 cDNA were determined. The length of the IL -2 was 522 bp ,encoding 154 amino aeids; however, the length of the IL-4 was 411 bp,encoding 133 amino acids. By blasting the homologous sequences in GenBank databases,the sequence of Rongchang pig interleukin -2 gene from lymphocyte is 99. 8% percent identity to the interleukin -2 genes previously cloned from other porcines;however, the sequence of Rongchang pig interleukin -4 gene is more than 98. 0%

关 键 词：荣昌猪 IL-2 IL-4 克隆 

分 类 号：S828.89[农业科学—畜牧学]