外燥对小鼠气管细胞凋亡与bcl-2基因及bax基因表达的影响  

Effects on Apoptosis and Expression of Bcl-2 and Bax Gene in Respiratory Intraepithelium of Mice with Outer-Dry

作　　者：丁建中[1] 龚权[1] 陈刚[2] 李军川[1] 陈庭煊[1] 李侃 

机构地区：[1]长江大学医学院,湖北荆州434000 [2]湖北中医学院,湖北武汉430061

出　　处：《时珍国医国药》2007年第1期15-16,共2页Lishizhen Medicine and Materia Medica Research

基　　金：湖北省自然科学基金资助项目(No.2004ABA185)

摘　　要：目的观察外燥对小鼠气管细胞凋亡与bc l-2基因及bax基因表达的影响。方法30只昆明种小鼠随机分为常温常湿组(A组),温燥组(B组),凉燥组(C组),每组10只。经处理后第14天用免疫组化法检测各组气管细胞凋亡率(%)、bc l-2、bax阳性指数(PI,%)、bc l-2/bax累积阳性指数(AI,%)与bc l-2:bax蛋白表达比值。结果3组小鼠气管细胞凋亡率均≤0.3%;B组与C组bc l-2 PI与AI均低于A组(P<0.01),B组、C组气管细胞bc l-2:bax蛋白比值中bax增加。结论外燥致气管细胞bc l-2表达降低、bc l-2:bax蛋白比值改变,bc l-2、bax阳性细胞以气道炎性病灶或纤毛缺损处多见,提示与外燥分子致病机制有关。Objective To investigate apoptosis and expression of bel - 2 gene, bax gene in respiratory intraepithelium of mice with outer- dry. Methods 30 kunming mice were divided into three groups, including group A （normal mice） , group B （warm -dry mice） , group C（ cool- dry mice） , 10 mice/group. Expression of bcl -2 gene, bax gene and apoptosis were measured on the 14th day. Results There was no significant difference for apoptosis among the three groups. Bcl -2 PI and AI in group B and C were markedly reduced compared with group A（ P 〈 0.01 ） , and bax gene increased in the ratio of bcl - 2 ： bax protein. Conclusion Out- er - dry can decrease the expression of bcl - 2 gene, change the ratio of bcl - 2 ： bax protein.

关 键 词：外燥 BCL-2基因 BAX基因 细胞凋亡 

分 类 号：R228[医药卫生—中医基础理论]