一种结肠炎相关的结肠癌模型的复制  

A novel model of colitis-related colorectal cancer

作　　者：李宇华[1] 刘莉[1] 冯娟[1] 王庆伟[1] 孙阳[1] 曹蔚[1] 王志鹏[1] 张蓉[1] 刘振国[1] 梅其炳[1] 

机构地区：[1]解放军第四军医大学药学系药理教研室,陕西省西安市710032

出　　处：《世界华人消化杂志》2007年第3期234-239,共6页World Chinese Journal of Digestology

摘　　要：目的:建立一个成功率高、简单、易行、价廉、短期成功的结肠炎相关的结肠癌模型.方法:5周龄ICR♂小鼠320只,随机分为实验组(1-7)和阴性(8)对照组.实验1-3组分别ip10,15,20mg/kg的1,2-二甲肼(DMH)1wk后,再给予小鼠饮用含20g/L的右旋葡聚糖苷钠(DSS)饮用水1wk.实验4-6组的小鼠仅通过ip给予10,15,20mg/kg的DMH.实验7组小鼠则只饮用含20g/L DSS的饮用水,第8组小鼠不给予任何处理.每周记录小鼠体质量及进食量,在4,9,13wk分别处死部分小鼠以观察小鼠的结肠炎癌变情况,并于20wk处死剩余小鼠.结果:ip DMH(10,15,20mg/kg)1wk后,再饮用含20g/L DSS的饮用水1wk.4wk可见40%小鼠便血.1wk后,便血消失.至9wk时,约有30%的小鼠再次出现便血体征.13wk时,约10%的小鼠出现脱肛现象.20wk时,40%左右的小鼠出现脱肛现象.在20wk100%♂ICR小鼠出现结肠腺癌,称取脾脏、胸腺以及结肠质量,结果发现,脾脏、结肠质量显著增加,胸腺质量明显减少,与对照组小鼠相比有显著性差异(脾脏质量:0.64±0.03,0.75±0.03,0.78±0.01gvs0.16±0.05g,P<0.01;结肠质量:0.93±0.06,0.96±0.02,0.01±0.06g vs 0.31±0.06g,P<0.01;胸腺质量:0.027±0.001gvs0.045±0.004g,P<0.05;0.026±0.002g vs 0.045±0.004g,P<0.05;0.016±0.003g vs 0.045±0.004g,P<0.01).结论:致癌剂DMH和致炎剂DSS可诱导ICR小鼠结肠腺癌的形成,较好的模拟了结肠炎癌变过程,方法价廉、简便、易行.AIM： To duplicate a colitis-related colorectal cancer model that is cheap, simple and accessible. METHODS： A total of 320 male ICR mice were randomly divided into 7 experimental groups （group A-G） and 1 control group. Groups A, B and C were given a single intraperitoneal injection of 1, 2-dimethylhydrazine （DMH） at the dose of 10, 15 and 20 mg/kg body weight, respectively, one week after injection, the animals in group A, B and C were treated with 20 g/L dextran sodium sulfate （DSS） in drinking water for consecutive 7 days. Group D, E and F were administered with DMH 10, 15 and 20 mg/kg body weight only, respectively. Group G was given 20 g/L DSS only. The weight and food intake of mice in each group were recorded every week. Several mice were killed to observed the progression to carcinoma of colitis in week 4, 9 and 13. All the mice were executed for observation in week 20. RESULTS： After administered with DMH and DSS, the bloody stools appeared in 40% mice in week 4, 30% in week 9, and rectal prolapse was observed in 10% mice in week 13, 40% in week 20. Within 20 weeks, all the mice, which had been given DMH and DSS, developed colonic adenocarcinoma. The weights of spleen and colon were increased significantly, while the weight of thymus was decreased obviously in group A, B, C in comparison with those in the control group （spleen： 0.64 ± 0.03, 0.75 ± 0.03, 0.78± 0.01 g vs 0.16±0.05 g, P 〈 0.01; colon： 0.93 ±0.06, 0.96 ± 0.02, 0.01± 0.06 g vs 0.31 ± 0.06 g, P 〈 0.01; thymus： 0.027 ± 0.001 g vs 0.045 ±0.004 g, P 〈 0.05; 0.026 ± 0.002 g vs 0.045 ± 0.004 g, P 〈 0.05; 0.016± 0.003 g vs 0.045 ± 0.004 g, P 〈 0.01）. CONCLUSION： DMH and DSS can effectively induce the formation of colonic cancer in ICR mice, which can well simulate the progression of colitis to carcinoma. The method used in this study is a cheap, simple and accessible.

关 键 词：结肠炎 结肠癌 ICR小鼠 1 2-二甲肼 右旋葡聚糖苷钠 

分 类 号：R-332[医药卫生] R735.35