CD117在急性髓细胞白血病中的表达特异度  

Enhanced myeloid specificity of CD117 in acute myeloid leukemia

作　　者：郑文娟[1] 李建勇[1] 吴雨洁[1] 宋君红[1] 杨慧[1] 

机构地区：[1]南京医科大学附属第一医院江苏省人民医院血液科,南京210029

出　　处：《临床血液学杂志》2007年第1期5-7,共3页Journal of Clinical Hematology

基　　金：江苏省医学领军人才项目资助

摘　　要：目的观察细胞表面分化抗原CD117在急性髓细胞白血病(AML)与急性淋巴细胞白血病(ALL)的表达差异及其意义,评价其作为髓系抗原的特异性。方法采用CD45/SSC双参数散点图设门法进行三色流式细胞术分析。直接免疫荧光标记法标记20种细胞表面分化抗原,经流式细胞仪测定,对286例白血病患者骨髓或外周血白血病细胞CD117及其他表面抗原的表达结果进行分析。结果CD117在ALL中表达率极低,仅占2%,在AML中表达率为56.9%,2者差异有统计学意义(P<0.05)。在AML各型中,CD117在M3亚型中的表达率最低,为23.1%。本组数据统计结果显示CD117的髓系特异度为0.98(SE=0.014),髓系敏感度为0.57(SE=0.04),统计结果表明CD117比CD13、CD33更具髓系特异性(P<0.05)。结论与CD13和CD33相比较,CD117在作为髓系标记的敏感度不如前两者高,但它更具有髓系特异度,因而可作为排除ALL及辅助诊断AML的标志。Objective：To investigate different expression of CD117 on acute myeloid leukemia （AML） and acute lymphoid leukemia （ALL） and its significance as a specific myeloid marker. Method：Immuno phenotype was analyzed by three-color flow eytometry using CD45/SSC gating. Result：CD117 was positive in only 2% patients with ALL, whereas 56.9% patients with AML. The expression of CD117 was lower in AML-M3 patients than in other subtype of AML. The results of statistical analysis showed that CD117 specificity to detect myeloid involvement was 0.98 with a sensitivity of 0.57. Conclusion：CD117 is a Useful marker for distinguishing myeloid from lymphoid leukemia, and CD117 provides greater specificity compared with CD13 and CD33 （ P d0. 005）.

关 键 词：白血病 髓细胞 急性 CD117 特异度 敏感度 

分 类 号：R733.71[医药卫生—肿瘤]