睫状神经营养因子突变体蛋白的活性研究  

Activity Study of Ciliary Neurotrophic Factor(CNTF)Mutant

作　　者：毕华[1] 袁力勇[1] 史新昌[1] 赵阳[1] 刘兰[1] 饶春明[1] 王军志[1] 

机构地区：[1]中国药品生物制品检定所生化室,北京100050

出　　处：《中国生物工程杂志》2007年第1期1-5,共5页China Biotechnology

基　　金：国家"863"计划资助项目(2003AA2Z3480)

摘　　要：为了进一步研究应用计算机分子模拟设计并表达纯化的睫状神经营养因子突变体蛋白的生物学活性,分别采用鸡胚背根神经节无血清培养法、TF-1细胞增殖法、正常小鼠减重法对其活性进行研究。结果表明突变体蛋白能促进鸡胚背根神经节的生长;促进TF-1细胞增殖,MTT测定法表明突变体蛋白与国际参考品相比,比活不低于2.0×106U/mg;使正常小鼠的体重减轻,摄食量减少,脂肪指数下降,并且体重的减轻与突变体蛋白的给药剂量呈现良好的剂量依赖关系,其ED50为:150.986μg/(kg·d)。以上实验表明CNTF突变体蛋白具有促神经生长、促TF-1细胞增殖和减重的生物学活性,为其进一步的应用和开发提供了依据。To study still further the activity of CNTF mutant designed by computer molecular modling, the methods of dissociated cultures of chick dorsal root ganglion,TF-1 prolification and the normal mice＇ weight loss tests were used. The results indicated that the mutant protein promoted the survival of dorsal root ganglion, induced TF-1 prolification and made the normal mice lose weight, decrease appetite and reduce fat index. The weight loss effect was dependant with its administration dosage, ED50 was 150.986μg/kg/d. To TF-1, the specific activity reached 2.0 × 10^6U/mg against international reference reagent. In a word, CNTF mutant had excel bioactivity. So it provided clues for its development and application.

关 键 词：睫状神经营养因子 突变体 活性研究 

分 类 号：Q954.67[生物学—动物学]