Nodule Formation and Development in Soybeans(Glycine max L.) in Response to Phosphorus Supply in Solution Culture  

作　　者：MIAO Shu-Jie QIAO Yun-Fa HAN Xiao-Zeng M. AN 

机构地区：[1]Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081 （China） [2]Key Labovatory of Soybean Biology of Ministry of Education, Harbin 150030 （China） [3]Graduate School of the Chinese Academy of Sciences, Beijing 100049 （China） [4]E. H. Graham Centre for Agricultural Innovation, NSW Department of Primary Industries ＆ Charles Sturt University, Wagga Wagga, NSW 2650 （Australia）

出　　处：《Pedosphere》2007年第1期36-43,共8页土壤圈（英文版）

基　　金：Project supported by the National Basic Research Program of China (Nos. 2005CB121101 and 2003CCB001);the Basic Research Program of Heilongjiang Province (Nos. GB05C201-01 and CC055303);the Field Station Foundation of the Chinese Academy of Sciences.

摘　　要：Phosphorus (P) is necessary for growth and nitrogen fixation, and thus its deficiency is a major factor limiting legume production in most agricultural soils. The effect of phosphorus supply on nodule development and its role in soybeans (Glycine max L.) was studied in a nutrient solution. Plants were inoculated with Bradyrhizobium japonicum and grown for 35 days in a glasshouse at a day and night temperature of 25℃and 15℃, respectively. Although increasing P supply increased the concentrations of P and N in the shoots and roots, the external P supply did not significantly affect the P concentration in the nodules, and the N fixed per unit nodule biomass decreased with increasing P supply. The nitrogen content in the shoots correlated well with the P content (r = 0.92**). At an inoculation level of 102 cells mL-1, the P supply did not affect the number of nodules; however, at inoculation levels of 103.5 and 105 cells mL-1, increasing P supply increased both the number and size of nodules. Irrespective of the inoculation level, increasing P supply increased the nodule biomass relative to the biomass of the host plant. It is suggested that the P deficiency specifically inhibited the nodule development and thereby the total N2 fixation.Phosphorus （P） is necessary for growth and nitrogen fixation, and thus its deficiency is a major factor limiting legume production in most agricultural soils. The effect of phosphorus supply on nodule development and its role in soybeans （Glycine max L.） was studied in a nutrient solution. Plants were inoculated with Bradyrhizobium japonicum and grown for 35 days in a glasshouse at a day and night temperature of 25℃ and 15℃, respectively. Although increasing P supply increased the concentrations of P and N in the shoots and roots, the external P supply did not significantly affect the P concentration in the nodules, and the N fixed per unit nodule biomass decreased with increasing P supply. The nitrogen content in the shoots correlated well with the P content （r=0.92＊＊）. At an inoculation level of 10^2 cells mL^-1, the P supply did not affect the number of nodules; however, at inoculation levels of 10^3.5 and 10^5 cells mL^-1, increasing P supply increased both the number and size of nodules. Irrespective of the inoculation level, increasing P supply increased the nodule biomass relative to the biomass of the host plant. It is suggested that the P deficiency specifically inhibited the nodule development and thereby the total N2 fixation.

关 键 词：inoculation level nitrogen fixation NODULATION PHOSPHORUS SOYBEANS 

分 类 号：S52[农业科学—作物学]