一株具有多途径氮代谢功能的荧光假单胞菌  

A STRAIN OF PSEUDOMONAS FLUORESCENS WITH MULTI-PATHS OF NITROGEN METABOLISM

作　　者：王晋宇[1] 范伟平[1] 李霜[1] 沈珈琦[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《土壤学报》2007年第1期144-149,共6页Acta Pedologica Sinica

基　　金：江苏省自然科学基金项目(BK2002019)资助

摘　　要：为研究全自养脱氮过程,采用斯凯尔曼亚硝化单胞杆菌培养基,从南京某城市污水处理厂序批式处理系统(SBR)中分离得到一株具有多途径氮代谢功能的荧光假单胞菌Pseudomonas fluorescensdN13。该菌株在有氧条件下,无有机碳源存在,能进行氧化NH3-N产能代谢生长;有有机碳源存在且NO2--N和NO3--N的浓度较高时,它能将NO2--N和NO3--N还原为NH3-N。在厌氧条件下,只有乙酸钠作碳源时,该菌株仍能进行硝酸亚硝酸还原(反硝化)作用。在这个具有多途径氮代谢功能的菌株细胞内,硝化反硝化过程耦合成为可能。To study on autotrophie removal of nitrogen from wastewater containing a high concentration of NH3-N, a strain of Pseudomonasfluorescens dN13 was isolated, by means of incubation in Skerman＇ s medium for Nitrosomona, from the sequence batch reactor system（SBR）of a wastewater treatment plant in Nanjing. Under aerobic condition, this strain could oxidize NH3-N into NO2^- -N and grow without organic carbon source; and reduce NO2^- -N and NO3^- -N into NH3-N with organic carbon source and adequate concentration of NO2^- -N and NO3^- -N, while under anaerobic condition, strain dN13 could still reduce NO2^- -N and NO3 -N into NH3-N with sodium acetate as the sole carbon source. In the cells of strain dN13 with such multi-paths of nitrogen metabolism, coupling of nitrification and denitrification is feasible.

关 键 词：荧光假单胞菌 有氧氨氧化 硝酸亚硝酸还原 多途径氮代谢 

分 类 号：X172[环境科学与工程—环境科学]