4种含RGD的短肽对细胞粘附作用的影响  

Effects of Motif RGD-containing Small Peptides on Cell Adhesion

作　　者：黄宜兵[1] 吴胜男[2] 徐力[1] 张学忠[1] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,长春130021 [2]华南师范大学激光生命科学研究所激光生命科学教育部重点实验室,广州510631

出　　处：《吉林大学学报（理学版）》2007年第1期128-134,共7页Journal of Jilin University:Science Edition

基　　金：教育部留学回国人员科研启动基金

摘　　要：从两方面对比研究了4种含Arg-Gly-Asp(RGD)的细胞粘附肽RGD,RGDS,RGD-(NH2)2即RGE-NH2和RGDS-NH2对细胞粘附的影响.一方面研究了固定在聚乙交酯丙交酯共聚物(PLGA)膜上的4种细胞粘附肽通过其细胞定向作用对大肠癌细胞(HCT-8)和人成骨肉瘤细胞(OS732)细胞粘附性的促进作用;另一方面研究了外源性细胞粘附肽对HCT-8和OS732在纤维连接蛋白(FN)上粘附的竞争性抑制作用.结果发现,除了RGD-(NH2)2,其他3种肽都具有明显抑制细胞粘附的作用且具有一定的浓度依赖关系,其中以RGDS和RGDS-NH2的能力最强,RGD稍弱.对于HCT-8细胞,3种肽的最大抑制率分别为53.3%,56.3%,37.5%;而对OS732细胞,3种肽的最大抑制率分别为50.9%,49.8%,34%.The cell adhesive effects of four peptides containing RGD, RGDS, RGD-（ NH2 ）2 and RGDS-NH2 on Human Intestines Cancer （HCT-8）, Human Osteogenic Sarconia （OS732） were investigated. First of all, these cell adhesion peptides were immobilized on PLGA blend membrane respectively and their effects on cell adhesion were investigated. The four peptides except RGD-（ NH2 ）2 on the membrane can promote the cell adhesion to the membrane. On the other hand, MTT assay was used to examine the adhesion of HCT-8 cells and OS732 cells on the Fibronectin （FN） after treatment with cellular adhesion peptide, we found that cellular adhesion peptides RGDS, RGDS-NH2, RGD significantly inhibited the adhesion of HCT-8 cells and OS732 cells on the FN in dose-dependent manner. RGD-（NH2）2 does not show an obvious ability to inhibit cell adhesion. The maximum percentages of inhibition were 53.3%, 56.3%, 37.5% on HCT-8 and 50.9%, 49.8% ,34% on OS732 by RGDS,RGDS-NH2 ,RGD, respectively.

关 键 词：细胞粘附肽 ARG-GLY-ASP 聚乙交酯丙交酯共聚物 大肠癌细胞 人成骨肉瘤细胞 纤维连接蛋白 

分 类 号：Q26[生物学—细胞生物学]