稠油降解菌的筛选及其生物表面活性剂的特性  

Screening of Biosurfactant-producing Thick Oil-degrading Bacteria and Its Characteristics of Biosurfactants

作　　者：伏亚萍[1] 李鱼[1] 王健[1] 曹周礼 段勇[1] 郭书海[3] 

机构地区：[1]吉林大学环境与资源学院,长春130012 [2]长春科技创业服务中心,长春130012 [3]中国科学院沈阳应用生态研究所,沈阳110016

出　　处：《吉林大学学报（理学版）》2007年第1期148-152,共5页Journal of Jilin University:Science Edition

基　　金：国家973计划项目基金(批准号:2004CB3418501)

摘　　要：添加稠油对土壤中土著微生物进行驯化,分离出33株能以稠油为惟一碳源生长的细菌,从中筛选出2株高效表面活性剂产生菌XJ1和SJ4,9株高效稠油降解菌.XJ1和SJ4可将发酵液的表面张力由72.4mN/m分别降到36.1mN/m和36.2mN/m;14d摇瓶油降解率分别为35.89%和31.59%,降解效率在各单菌中最高.同时研究了发酵液中XJ1和SJ4的生长量与其生物表面活性剂产生情况之间的关系,经红外光谱分析初步确定两种生物表面活性剂均为糖脂类化合物.Indigenous microbial strains of the soil were habituated by adding thick oil intentionally. Thirty-three bacterial strains were isolated with thick oil as the carbon source alone. Two higher biosurfactant produced strains and nine higher thick oil-degrading strains were screened from all the isolates. The biosurfactants produced by strain XJ1 and SJ4 in the fermentation broth could reduce the surface tension of water from 72.4 mN/m to 36.1 mN/m and 36.2 mN/m respectively. In the process of inoculating the two strains, the rates of thick oil-degradation were 35.89% and 31.59% after treatment for 14 days. For strains XJ1 and SJ4, the relation between biology quantity and the surface tension of fermentation broth was studied simultaneously. Infrared spectral analytic results show the biosurfactants are both kinds of glycolipid.

关 键 词：生物表面活性剂 稠油降解菌 土壤微生物修复 稠油 

分 类 号：X132[环境科学与工程—环境科学]