转TrxS基因对啤酒大麦种子贮藏蛋白含量的影响  

Effect of Trans-gene TrxS on the Content of Storage Protein of Malting Barley Seeds

作　　者：卫丽[1] 尹钧[1] 黄晓书[1] 孔维威[1] 邓德芝[1] 

机构地区：[1]河南农业大学国家小麦工程技术研究中心,河南郑州450002

出　　处：《麦类作物学报》2007年第1期26-29,共4页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(30370877);国家转基因植物研究与产业化专项(JY03-B-19)

摘　　要：为探讨导入TrxS基因对大麦籽粒氮代谢的作用,以转TrxS基因啤酒大麦纯合株系为材料,分析了TrxS基因的导入对大麦籽粒蛋白酶活性、醇溶蛋白和谷蛋白含量的影响。结果表明,转基因大麦籽粒的蛋白酶活性增加,发芽第3-5 d转基因大麦种子的蛋白酶活性分别比对照增加42.86%、19.35%和25.76%;发芽第2-4 d醇溶蛋白和谷蛋白的降解平均比对照下降快5.92%和7.10%。In order to understand the function of TrxS gene on N metabolism of barley seeds, this pa- per analyzed the effect of TrxS gene on protease activity and contents of alcohol-soluble proteins of barley seeds during germination period by using TrxS transgenic barley lines. The results showed that the activities of protease in transgenic seeds were 42. 86%,19.35% ,25. 76% higher than that of nontransgenic seeds during 3～5 germinating days, respectively; The degradation of hordein and glutelin of transgenic seeds were 5.92% and 7.10% quicker than that of non-transgenic seeds in average.

关 键 词：大麦 转TrxS基因 蛋白酶 醇溶蛋白 谷蛋白 

分 类 号：S512.3[农业科学—作物学] S330