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作 者:刘成[1] 杨足君[1] 冯娟[1] 周建平[1] 任正隆[1]
机构地区:[1]电子科技大学生命科学与技术学院,四川成都610054
出 处:《麦类作物学报》2007年第1期35-40,共6页Journal of Triticeae Crops
基 金:国家自然科学基金项目(30170502和30170579)
摘 要:为了筛选黑麦染色体组特异性分子标记,以荆州黑麦、秦岭黑麦、森林黑麦、非洲黑麦、普通小麦中国春、R25、R111和MY11为材料,用A-M组142个10碱基随机引物进行RAPD扩增。发现引物M04可以在所有黑麦中扩出一条长1 143 bp(经测序)的特异片段OPM041143,而供试小麦材料均未扩出该片段。根据OPM041143设计特异引物M4-F和M4-R。利用M4-F和M4-R对含黑麦染色体的材料和小麦族其它物种进行扩增,结果表明,只有含黑麦染色体的材料均可以扩出一条分子量为1 082 bp的DNA带,命名为PSCM1082。进而利用一套中国春-Imperial黑麦二体附加系和几个小麦-黑麦染色体6R衍生系进行扩增,发现仅含6R染色体的材料能扩增出PSCM1082,这说明PSCM1082是黑麦6R染色体所特有。黑麦6R染色体特异PCR标记PSCM1082的发现,对于快速跟踪检测导入小麦中的6R染色体具有实用价值,可以应用于小麦育种工作中。In order to find out special molecular marker of genome of rye, random amplified polymorphic DNA (RAPD) analysis was performed on Secale cereale, S. creale. L. Qingling, S. silvestre, S. africanum, common wheat cultivar Chinese Spring, R25, Rlll and MY11 with 142 random 10-based primers. A chromosome-specific polymorphic DNA segment, OPM041143, was obtained in all of the rye derived lines, but there was no OPM041143 in all wheat materials tested in this study. A pair of special primers M4-F and M4-R was designed based on OPM041143 in order to obtain a sequence charac- tered amplified region (SCAR) marker. The SCAR-PCR was performed on materials containing rye chromosome and some other Triticeae species using M4-F and M4 R. The result showed that special DNA segment of 1082 bp only obtained in all material containing rye chromosome, named PSCM1082 and there was no PSCM1082 in other material of Triticeae. Furthermore, PCR analysis was performed on a set of Chinese Spring-S. cereale addition lines and several wheat - rye 6R chromosome derivatives, the result showed that PSCM1082 could be amplified in all materials contain 6R chromosome. Therefore, PSCM1082 is a chromosome-specific polymorphic DNA segment for rye chromosome 6R, it could be used as a molecular marker for detection of 6R chromosome of rye in wheat.
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