多穗柯中二氢查耳酮的提取、纯化与含量测定  

Isolation and determination of dihydrocharcone in lithocarpus polysachyus pehd

作　　者：于丽静[1] 宁正祥[1] 于丽伟[2] 于希福[3] 

机构地区：[1]华南理工大学轻工与食品学院,广州510640 [2]吉林农业大学动物科技学院,长春130118 [3]对外经济贸易大学外语学院,北京100029

出　　处：《中国食品添加剂》2007年第1期176-179,175,共5页China Food Additives

基　　金：广州市科技局项目(2006J1-C0251);广东省科技计划支持项目(2005A20303002)

摘　　要：以多穗柯(LithocarpuspolystachyusRehd)叶为原料,研究了二氢查耳酮提取及纯化的适宜条件。结果表明,二氢查耳酮提取的适宜条件为:以1∶8的料水比,在80℃条件下提取60min;二氢查耳酮纯化的适宜条件为:以聚合氯化铝为絮凝剂,在pH值为7·5,温度为55℃时絮凝40min;以ADS-7树脂为吸附剂,以100mg/mL为上样液浓度,80%乙醇为洗脱液浓度。二氢查耳酮的提取率达5·26%。用高效液相色谱法检测,二氢查耳酮含量为98·37%。Dihydrocharcone as the target, studied the proper process for extracting and purifying Dihydrocharcone from Lithocarpus polystachyus Rehd. The result indicates the best extracting conditions are： when the solvent is water the weight of the water is 8 times that of the Lithocarpus polystachyus Rehd, the temperature of the water is 60℃, the period of the extraction is 60 minutes; the best flocculate is Polyaluminum Chloride, the best sorbent is ADS - 7 macroporous resin, then the content of dihydrocharcone can reach 5. 26%. Use HPLC to check, the pure of the dihydrocharcone is 98. 37%.

关 键 词：多穗柯 二氢查耳酮 高效液相色谱 

分 类 号：TS028.96[轻工技术与工程]