KDR启动子驱动双自杀基因诱导胃癌细胞凋亡的体内实验研究  

In vivo study on the cell apoptosis of gastric cancer cells induced by adenovirus mediated fusion gene system driven by KDR promoter

作　　者：黄宗海[1] 李强[1] 苏国强[1] 俞金龙[1] 厉周[1] 周广军[1] 

机构地区：[1]南方医科大学珠江医院普外科,广东广州510280

出　　处：《中国现代医学杂志》2007年第1期17-19,23,共4页China Journal of Modern Medicine

基　　金：国家863计划项目(2001AA217171);广东省自然科学基金项目(013072)

摘　　要：目的探讨KDR启动子驱动双自杀基因体系对胃癌细胞凋亡和增值的影响。方法建立小鼠SCG7901胃癌细胞膜型,随机分为4组。治疗结束后,称取瘤重并计算抑瘤率。用电镜和TUNEL法检测双自杀基因体系作用下SCG7901胃癌细胞的凋亡。结果实验组(B组)肿瘤体积逐渐缩小,而对照组(A、C和D组)肿瘤体积逐渐增大。透射电镜下可见实验组出现凋亡细胞,TUNEL法显示实验组肿瘤细胞凋亡率显著高于对照组,P<0.01。结论KDR启动子驱动双自杀基因体系对裸鼠皮下移植瘤有明显的生长抑制作用,其机制可能与双自杀基因体系诱导肿瘤细胞凋亡有关。[Objective] To observe the apoptosis and cell proliferation induced by by adenovirus mediated fusion gene system driven by KDR promoter. [Methods] Human stomach cancer cell line （SCG7901） was seeded in the subcutaneous and implanted in a xenograft model by using nude mice, Then they were randomly divided into four groups. Tumor size was measured in 2 dimensions at the end of treatment. Tumor sections were examined by electron microscope and TUNEL method. [Results] Tumors decreased in size during treatment of double suicide gene system （B group）, but increased in size in the control groups （A, C, D group）. There were a lot of cells underwent apoptosis in the treated groups. TUNEL method demonstrated that apoptotic rate in treated groups was significantly higher than that in the untreated control groups, （P 〈0.01）. [Conclusions] Usion gene system driven by KDR promoter can significantly inhibit the tumor growth in nude mice bearing SCG7901 cells. Its mechanism may be closely related to inducing apoptosis in tumor cells.

关 键 词：胃肿瘤 细胞凋亡 自杀基因治疗 裸鼠 

分 类 号：R730.21[医药卫生—肿瘤]