汞对大鼠外周神经影响的形态学研究  

Morphology study on the effect of Mercury on the peripheral nervous system of rat

作　　者：徐旭东[1] 郭启明[1] 周永田[1] 闫伟[2] 徐明怡[1] 谢林[1] 郁东[1] 秦占霞[1] 

机构地区：[1]山东省职业卫生与职业病防治研究院,山东济南250002 [2]山东中医药大学,山东济南250002

出　　处：《中国现代医学杂志》2007年第1期40-43,共4页China Journal of Modern Medicine

基　　金：山东省医学科学院资助项目;编号为2004-0023

摘　　要：目的观察HgCl2对胫神经的影响。方法SD大鼠70只,35♀,35♂,体重160￣200g。分为4组;急性毒理学试验组40只(20♀,20♂)。按霍恩氏法求出经口灌胃LD50为61.8mg/kg。大剂量染汞组10只(5♀,5♂),按17mg/kg(1/4LD50)经口灌胃HgCl2溶液,每天1次。小剂量染汞组10只(5♀,5♂),按8.5mg/kg(1/8LD50)经口灌胃HgCl2溶液,每天1次。对照组10只(5♀,5♂),每天1次经口灌胃2mL生理盐水。对照组与染汞组连续灌胃(20±4)d,制成亚急性汞中毒模型,然后用二巯基丙磺酸钠(DMPS)驱汞两个疗程(驱3d,休4d为1个疗程)。分别于造模后和驱汞后用透射电镜观察大鼠胫神经超微结构的变化。结果大剂量染汞组中有7只出现汞中毒,其中1只雄鼠出现疼痛表现。小剂量组中有6只出现汞中毒,均无疼痛表现。电镜放大4000￣20000倍见;汞中毒大鼠胫神经出现脱髓鞘改变,疼痛大鼠改变更显著,DMPS驱汞2个疗程未能阻止脱髓鞘病变。结论亚急性HgCl2中毒可以导致SD大鼠胫神经脱髓鞘病变。[Objective] To observe the effect of Mercury on the peripheral nervous system. [Method] Seventy SD rats （35 ♀,35 ♂, weighing 160-200 g） were divided into 4 groups. Group 1 include 40 rats （20 ♀,20 ♂ ） which were used to find out the acute toxicity of HgCL2 and the oral LD50 is 68.1 mg/kg. Group 2 include 10 rats （5 ♀,5 ♂ ） which received HgCL2 juice at a dose of 17 mg/kg （1/4 LD50） by gavage daily. Group 3 include 10 rats （5 ♀,5 ♂） which received HgCL2 juice at a dose of 8.5 mg/kg （1/8 LD50） by gavage daily. Group 4 include 10 rats （5 ♀,5 ♂ ） which received 0.9% saline 2 mL by gavage daily. The duration of experiment is （20±4） days. After the modal had been set up, the injection of sodium dimeroaptopropane sulfonate （DMPS） was administered by abdomen injection at a dose of 28 mg/kg for 2 periods. Then the rats were dissected and the nerve of tibialis was observed by electric microscope （enlarging 4 000-20 000 times）. [Result] Seven rats in group 2 （7/10） were poisoned and one of them got pain syndrome. Six rats in group 3 （6/10） were poisoned. The tibialis nerve of poisoned rat is myelioclasis and more serious in pain rat. To administer DMPS do not stop the change. [Conclusion] Sub-acute poisoning of HgCL2 results in myelinoclasis of tibialis nerve of SD rat.

关 键 词：汞中毒 周围神经 脱髓鞘 疼痛 大鼠 

分 类 号：R-332[医药卫生]