高压氧联合化疗药物对结肠腺癌细胞CT26小鼠移植瘤生长的影响  

Effects of hyperbaric Oxygen Combined with Chemotherapy on the Proliferation of Colorectal Carcinoma in Vivo

作　　者：夏扬[1] 汪爱国[2] 张伟[2] 黄平[3] 黄培林[2] 张丽达[3] 

机构地区：[1]东南大学附属中大医院康复科,南京210009 [2]东南大学医学院病理学系,南京210009 [3]东南大学附属中大医院高压氧科,南京210009

出　　处：《现代生物医学进展》2006年第12期27-30,共4页Progress in Modern Biomedicine

基　　金：南京市社会发展科技计划(200401070-3);江苏高校高新技术产业发展项目(HJ03-059)资助

摘　　要：目的:探讨高压氧联合化疗药物对结肠腺癌细胞CT26小鼠移植瘤生长的影响。方法:建立小鼠结肠腺癌移植瘤模型,观察0．2 Mpa HBO及联合化疗药物5-FU或紫杉醇对荷瘤小鼠肿瘤生长的影响,计算肿瘤抑制率;用流式细胞仪观察0．2 Mpa HBO对CT26细胞周期的影响。结果:动物实验结果显示:HBO组小鼠肿瘤体积抑制率为:22．39％,肿瘤质量抑制率为:25．77％;5-FU组分别为:42．38％,43．61％;5-FU+HBO组分别为:72．10％,71．47％;紫杉醇组分别为:26．31％,23．04％;紫杉醇+HBO分别为: 33．24％,30．96％。5-FU+HBO组与5-FU组及HBO组相比较有显著性差异(p<0．01);紫杉醇+HBO组与紫杉醇组及HBO组相比差异不明显。流式细胞仪检测结果显示:0．2 Mpa HBO诱导CT26细胞在S期积蓄(G0／G1期(55．89％)、S期(40．79％)、G2／M期(3．32％))。结论:0．2 Mpa HBO可抑制了CT26结肠腺癌小鼠移植瘤的生长,并能增强细胞周期特异性化疗药物的敏感性。Objective： To study the effects of hyperbaric oxygen （HBO） or combined with chemotherapy （5-FU or paclitaxel）on the proliferation of colon adenocardnoma cell GT26 transplanted in mice. Methods： The cell cycle of GT26 under hyperbaric oxygen was examined with flow cytometry; BALB/c mouse tumor models were establishedand and the effects of 0.2 Mpa HBO or combined with chemotherapy （5-FU or paclitaxel） on the growth of tumors were observed. The tumor inhibitory ratio was calculated. Results： The volume inhibitory ratios or weight inhibitory ratios in different groups were as follows： HBO group （22.39%,25.77%） ; 5-FU group （42.38%,43.61%） ; 5-FU＋HBO group （72.10%,71.47%） ; Paclitaxel group （26.31%, 23.04%） ; Paclitaxel＋HBO group （33.24,30.96%）. The tumor inhibitory ratio in 5-FU＋HBO group was higher than that in 5-FU group or HBO group,but there was no difference between Paclitaxel＋HBO group and Paclitaxel group or HBO group. Conclusion： These results show that HBO can improve the effect of cell cycle specific antitumor drugs, HBO itself may inhibit the growth of transplanted mouse tumor. HBO may induce cancer cells into cell cycle and accumulate in S phase in vitro; and inhibit the growth of epidermic tumors in vivo.

关 键 词：高压氧 化疗药物 结肠癌细胞 移植瘤 小鼠 

分 类 号：R73-36[医药卫生—肿瘤] R735.3[医药卫生—临床医学]