多种雷公藤单体抑制HL-60细胞腺苷脱氨酶活性  

Various monomers of Tripterygium wilfordii effecting adenosine deaminase activity and inducing HL-60 cell apopotosis

作　　者：王莉[1] 张腾龙[1] 范华骅[2] 陈波斌[1] 谢彦晖[1] 

机构地区：[1]复旦大学附属华山医院血液科,上海200040 [2]上海市血液中心,上海200051

出　　处：《复旦学报（医学版）》2007年第1期107-110,共4页Fudan University Journal of Medical Sciences

摘　　要：目的研究多种雷公藤单体对HL-60细胞腺苷脱氨酶(ADA)的影响和诱导细胞凋亡作用。方法分别用雷公藤春碱、雷公藤内酯甲、雷公藤乙素、雷公藤黄素、雷公藤呋喃南蛇碱、雷公藤定碱(10mg/L、1mg/L、0.1mg/L)与HL-60细胞共孵育24h后测定ADA活性,选出抑制作用最强的雷公藤内酯甲。将HL-60细胞与雷公藤内酯甲10mg/L、1mg/L、0.1mg/L以及0.1mg/L雷公藤内酯甲+腺苷0.5mmol/L、腺苷0.5mmol/L共孵育24h后测定细胞凋亡率。结果①雷公藤内酯甲和雷公藤呋喃南蛇碱均能明显抑制HL-60细胞ADA活性(P<0.01),抑制作用与浓度成正比;雷公藤春碱仅在10mg/L浓度时有明显的ADA抑制作用(P<0.01);雷公藤黄素、雷公藤定碱与雷公藤乙素无明显ADA抑制作用(P>0.05)。②HL-60细胞与雷公藤内酯甲10mg/L、1mg/L作用均可见明显的凋亡,单加内酯甲0.1mg/L时或单加腺苷时与对照组比较凋亡率无显著差异,但同时再加入腺苷有很高的凋亡率,两者有协同作用。结论雷公藤内酯甲、雷公藤呋喃南蛇碱和雷公藤春碱等雷公藤单体在一定浓度下对HL-60细胞ADA有抑制作用,可能是ADA一种抑制剂。雷公藤内酯甲在抑制细胞ADA活性的同时也造成明显的细胞凋亡。Purpose To investigate the effect of various monomers of Tripterygium wilfordii on adenosine deaminase（ADA）activity in HL-60 cell and their function of inducing cellular apoptosis. Methods HL-60 cell was incubated with different monomers of Tripterygium wilfordii at different concentrations（10 mg/L, 1 mg/L,0. 1 mg/L） for 24 hours to detect the ADA activity. Wilforlide A, which was the most potent inhibitor to ADA activity,was selected out for future investigation. HL-60 cell was cultured with different concentrations of Wilforlide A（10 mg/L, 1 mg/L,0. 1 mg/L）,or with 0.1 mg/L Wilforlide A plus 0.5 mmol/L adenosine,or 0. 5 mmol/L adenosine alone for 24 h to test their effect on apoptotic rate. Results ①Both Celafurine and Wilforlide A could obviously inhibit ADA activity in HL-60 cell（P〈0.01）, moreover, their inhibitory ability was in direct proportion to their concentrations. While the inhibitory function of Wilfortrine could only be detected at the concen- tration of 10 mg/L（P〈0.01）. 3-epikatonic acid, Wilfordine and Tripdiolide showed no obvious inhibitory function on ADA activity in HL-60 cell（P〉0.05）. ②The obvious apoptosis of HL-60 cell could be detected after incubated with Wilforlide A at 10 mg/L or 1 mg/L. It showed no marked difference in HL-60 cell apoptosis when incubated only with 0.1 mg/L of Wilforlide A or only with adenosine com-pared to control groups. But the higher apoptosis could be observed when both Wilforlide A and adeno- sine were added to HL-60 cell. So, Wilforlide A and adenosine probably had synergistic effect on cellular apoptosis. Conehasions Some monomers of Tripterygium Wilfordii, including Wilforlide A, Celafurine and Wilfortrine,could inhibit the activity of ADA at certain concentrations. They were probably one kind of inhibitors to ADA. Meanwhile, Wilforlide A could reduce obvious cellular apoptosis while inhibited ADA activity in HL-60 cell.

关 键 词：雷公藤单体 腺苷脱氨酶 HL-60细胞 

分 类 号：R733.7[医药卫生—肿瘤]